Dichloromethane

Formic acid, free cholesterol enzymatic test kit, trimethylamine, pyridine, sodium sulphate, palmitic acid, and palladium/carbon were purchased from FUJIFILM WAKO Chemicals (Osaka, Japan). Acetone, chloroform, ethanol, filipin complex, 1-hydroxytriazole, Ham′s F-12 medium, iodine, pivaloyl chloride, serine, and toluene were purchased from Nacalai tesque (Kyoto, Japan). Acetonitrile, methanol-d₄, and chloroform-d were purchased from Kanto Kagaku (Tokyo, Japan). Acetyl chloride, dichloromethane, 1, 3-dicyclohexylcarbodiimide, imidazole, N-methylmorpholine, L-serine benzyl ester hydrochloride, and phosphorus trichloride were purchased from Tokyo chemical industry Co. (Tokyo, Japan). NBD-F was purchased from DONJINDO laboratories (Kumamoto, Japan). Choline tosylate was purchased from Toronto Research Chemicals (North York, ON, Canada). Foetal bovine serum was purchased from Gibco (Grand island, NY, USA). SPC and Lyso-SM (d17:1) were purchased from Avanti polar lipids, Co. Ltd. (Alabaster, AL, USA). Ultrapure water was prepared with PURELAB ultra (Organo Co. Ltd., Tokyo, Japan) and used in all MS analyses.

Hoechst 33342 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Invitrogen (MA, USA). Dimethyl sulfoxide (DMSO) and epidermal growth factor (EGF) were purchased from Sigma-Aldrich (MI, USA). Chloroform (CHCl₃), deuterated Chloroform (CDCl₃), dichloromethane (CH₂Cl₂), Dimethyl sulfoxide, ethyl acetate (CH₃COOC₂H₅), hexane (C₆H₁₄), isotonicotinoyl chloride hydrochloride (C₆H₄ClNO), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (C₈H₁₇N₃·HCl) (ECDI), dichloromethane (CH₂Cl₂) and silica gel 7734 were purchased from Tokyo Chemical Industry (Tokyo, Japan).