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Type 4 collagen coated plates

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Type IV collagen-coated plates are a type of cell culture substrate. They are coated with type IV collagen, a key component of the extracellular matrix. These plates provide a surface that supports the attachment, growth, and differentiation of various cell types.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Hepg2, by American Type Culture Collection (2 mentions) Tissue culture treated plastic ware, by Corning (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Tetracycline, by Merck Group (1 mentions)

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Collagen IV (188 citations) Collagen type IV (90 citations) Type IV (10 citations) Collagen-coated plates (2 citations)

2 protocols using type 4 collagen coated plates

1

Cell Culture Protocol for 293T and HepG2

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293T (American Tissue Culture Collection, ATCC® Number: CRL-3216TM, Manassas, VA) and HepG2 cell lines (American Tissue Culture Collection, ATCC® Number: HB-8065™, Manassas, VA) were maintained in Dulbecco’s modified Eagle medium (DMEM; ThermoFisher, Waltham, MA) base medium supplemented with 10% (vol/vol) fetal bovine serum (FBS) (Sigma Aldrich, St Louis, MO). 293T cells were grown on tissue culture-treated plastic ware (Corning Inc., Corning, NY) and HepG2 cells on type IV collagen-coated plates (Sigma Aldrich, St Louis, MO).
Liu Y., Cafiero T.R., Park D., Biswas A., Winer B.Y., Cho C.H., Bram Y., Chandar V., Connell A.K., Gertje H.P., Crossland N., Schwartz R.E, & Ploss A. (2023). Targeted viral adaptation generates a simian-tropic hepatitis B virus that infects marmoset cells. Nature Communications, 14, 3582.
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2

Culturing Human Cell Lines for Research

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In all, 293 T (American Tissue Culture Collection, ATCC® Number: CRL-3216TM, Manassas, VA) and HepG2 cells (American Tissue Culture Collection, ATCC® Number: HB-8065™, Manassas, VA) were maintained in Dulbecco’s modified Eagle medium (DMEM; ThermoFischer, Waltham, MA) base medium supplemented with 10% (vol/vol) fetal bovine serum. HepG2.2.15 cells7 (link) (kindly provided by Dr Christoph Seeger, Fox Chase Center) were maintained in DMEM/10% FBS media containing tetracycline (Sigma Aldrich, St Louis, MO) at 10  μg/ml. 293 T cells were grown on tissue culture-treated plastic ware (Corning Inc., Corning, NY) and HepG2 and HepG2.2.15 cells on type IV collagen-coated plates (Sigma Aldrich, St Louis, MO).
Winer B.Y., Huang T.S., Pludwinski E., Heller B., Wojcik F., Lipkowitz G.E., Parekh A., Cho C., Shrirao A., Muir T.W., Novik E, & Ploss A. (2017). Long-term hepatitis B infection in a scalable hepatic co-culture system. Nature Communications, 8, 125.
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