Primary antibodies used in this work included a rabbit polyclonal antibody raised against purified SINV C protein generated in our laboratory. Rabbit polyclonal anti-TIA-1 (C-20): sc-1751 and rabbit polyclonal anti-eIF2α antibodies were purchased from Santa Cruz Biotechnology. Rabbit polyclonal anti-eIF2D antibody was purchased from Proteintech, rabbit polyclonal anti-eIF2A antibody was purchased from Bethyl Laboratories Inc., and a rabbit polyclonal antibody raised against phospho-eIF2α (serine 51) was purchased from Cell Signaling Technology.
Rabbit polyclonal anti eif2d antibody
Manufactured by Proteintech
The Rabbit polyclonal anti-eIF2D antibody is a laboratory reagent used to detect the eIF2D protein. It is produced by immunizing rabbits with a specific antigen and purifying the resulting polyclonal antibodies. This antibody can be used in various immunoassay techniques to identify and quantify the eIF2D protein in biological samples.
Automatically generated - may contain errors
2 protocols using rabbit polyclonal anti eif2d antibody
1
Antibody Reagents for SINV Study
2
Antibody Detection Protocol for SINV
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The following primary antibodies were used: rabbit polyclonal antibody against purified SINV C protein, generated in our laboratory; rabbit polyclonal anti-firefly luciferase (ab 21176, Abcam); goat polyclonal anti-TIA-1 (C-20) sc-1751 and rabbit polyclonal anti-eIF2α antibodies (Santa Cruz Biotechnology); mouse monoclonal anti-α-tubulin (T5168, Sigma-Aldrich); rabbit polyclonal anti-eIF2D antibody (Proteintech Group); rabbit polyclonal anti-eIF2A (Bethyl Laboratories Inc.); and rabbit polyclonal antibody anti-phospho-eIF2α (serine 51) (Cell Signaling Technology). Anti-rabbit or anti-mouse IgG secondary antibodies coupled to peroxidase (Amersham Biosciences) were used at a 1:5000 dilution.
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