4kx4k fei eagle ccd camera

Manufactured by Thermo Fisher Scientific

The 4Kx4K FEI Eagle CCD camera is a high-resolution digital imaging device designed for electron microscopy applications. It features a 4,096 x 4,096 pixel sensor with a 16-bit depth, providing detailed imaging capabilities. The camera is optimized for use with FEI electron microscopes, delivering high-quality image capture and data acquisition.

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Lab products found in correlation

Lacey substrate, by Ted Pella (2 mentions) 300 mesh cu grid, by Ted Pella (2 mentions) Vitrobot mark 4, by Thermo Fisher Scientific (1 mentions) Tecnai 12 g2 twin tem, by Thermo Fisher Scientific (1 mentions) Gatan model 626, by Ametek (1 mentions) Tecnai12 g2 spirit twin tem, by Thermo Fisher Scientific (1 mentions)

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Eagle CCD camera CCD camera

3 protocols using 4kx4k fei eagle ccd camera

Negative-Staining and Cryo-TEM Imaging

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Negative-staining transmission
electron microscopy (TEM) was performed by applying a drop (3 μL)
of sample to a glow-discharged TEM grid (carbon-supported film on
300-mesh Cu grids, Ted Pella, Ltd.). After 30 s the excess liquid
was blotted, and the grids were stained with 2% uranyl acetate for
30 s and allowed to dry in air. Imaging was carried out using a FEI
Tecnai 12 G2 Twin TEM operated at 120 kV. The images were recorded
by a 4Kx4K FEI Eagle CCD camera using the TIA software. Direct imaging
of samples by cryo-TEM was performed as described elsewhere.³⁹ A 3 μL sample was applied onto a glow-discharged
300-mesh copper TEM grid coated with a holey carbon film (Lacey substrate,
Ted Pella, Ltd.). The excess liquid was blotted and the specimens
were vitrified by rapid plunging into liquid ethane precooled by liquid
nitrogen using a Vitrobot Mark IV (FEI). We then transferred the vitrified
samples into a cryo specimen holder (Gatan model 626; Gatan Inc.)
and imaged them at −177 °C using a Tecnai 12 G2 Twin TEM
(FEI), operated at an acceleration voltage of 120 kV in low-dose mode.
Images were recorded with a 4Kx4K FEI Eagle CCD camera. TIA (Tecnai
Imaging & Analysis) software was used to record the images.

Szebeni J., Kiss B., Bozó T., Turjeman K., Levi-Kalisman Y., Barenholz Y, & Kellermayer M. (2023). Insights into the Structure of Comirnaty Covid-19 Vaccine: A Theory on Soft, Partially Bilayer-Covered Nanoparticles with Hydrogen Bond-Stabilized mRNA–Lipid Complexes. ACS Nano, 17(14), 13147-13157.

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Transmission Electron Microscopy of RGS L106R

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RGS L106R was incubated at a concentration of 8 μM in 50 mM Tris HCl pH 7.4, 150 mM NaCl, 5 mM β-mercaptoethanol at 25°C for 12 hours. The aggregation reaction solution was diluted 10x in the same buffer prior to sample preparation. A drop of 3-5 μL sample was applied to a glow discharged TEM grid (carbon supported film on 300 mesh Cu grids, Ted Pella, Ltd.). After 30 sec the excess liquid was blotted, the grids were stained with 2% uranyl acetate for 30-60 sec, blotted and allowed to dry in air. The samples were examined using FEI Tecnai 12 G2 TWIN TEM operated at 120 kV. The images were recorded by a 4K
x 4K FEI Eagle CCD camera.

Garfagnini T., Harries D., & Friedler A. (2020). Osmolytes and Macromolecular Crowders Diversely Affect the Aggregation of the Cancerrelated L106R Mutant of the Axin RGS Domain.

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Cryo-TEM Imaging of Liposomes

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А drop (3.5 μl) of a liposome suspension was applied to a glow discharged TEM grid (300-mesh Cu grid) coated with a holey carbon film (Lacey substrate, Ted Pella, Ltd.). The excess liquid was blotted, and the specimen was vitrified by a rapid plunging into liquid ethane pre-cooled with liquid nitrogen using Vitrobot Mark IV (FEI). The vitrified samples were transferred to a cryo-holder (Gatan model 626), and examined at -177 C using a FEI Tecnai 12 G 2 Spirit TWIN TEM. The images were recorded (4Kx4K FEI Eagle CCD camera) at 120 kV in low-dose mode. TIA (Tecnai Imaging & Analysis) software was used to record the images and to measure the thickness of the liposome's membrane.

Deyev S., Proshkina G., Baryshnikova O., Ryabova A., Avishai G., Katrivas L., Giannini C., Levi-Kalisman Y, & Kotlyar A. (2018). Selective staining and eradication of cancer cells by protein-carrying DARPin-functionalized liposomes. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130.

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