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Leica micro cdmi 6000b

Manufactured by Leica camera
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The Leica/Micro CDMI 6000B is a high-performance microscope designed for advanced imaging and analysis applications. It features a modular design, allowing for customization and adaptation to various research and industrial needs. The microscope provides exceptional optical performance, enabling users to obtain detailed and accurate images of their specimens.

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Lab products found in correlation

Ab72583, by Abcam (2 mentions) Mab377, by Merck Group (2 mentions) Cbl1512, by Merck Group (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594, by Thermo Fisher Scientific (2 mentions) G3893, by Merck Group (2 mentions)

2 protocols using leica micro cdmi 6000b

1

Immunohistochemical Mapping of GABA in Spinal Cord

Cited 1 time
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The distribution of GABA in the SDH was determined by immunohistochemistry. Rats were perfused intracardially with 4% paraformaldehyde in 0.1 M phosphate buffer, and the L4–L5 segments of the spinal cord were removed, postfixed in the same solution for overnight, and cryoprotected with 30% sucrose in PBS for 2 days. Cryostat sections (35µm thickness) were incubated overnight at 4 °C with rabbit anti-GABA (1:1000; a gift from Dr. Yuan Zhu, Department of Medicine, University of Michigan, MI), mouse anti-GFAP antibody (1 : 2000, cata# G3893, Sigma, St. Louis, MO), mouse anti-OX42 antibody (1:1000, cata# CBL1512, Millipore, Billerica, MA), mouse anti-NeuN monoclonal antibody (A60) (1 : 5000, cata# MAB377, Millipore, Billerica, MA), or rabbit anti-Wnt5a (1:1000, cata# ab72583, Abcam, Cambridge, MA) followed by fluorescent IgG (Alexa Fluor 488, 1:1000 or Alexa Fluor 594, Molecular Probes, Eugene, OR, USA) for 2 hours at room temperature. Fluorescence images were captured by a fluorescent microscopy (Fluorescent M Leica/Micro CDMI 6000B)29 (link), 88 (link).
Kanda H., Kanao M., Liu S., Yi H., Iida T., Levitt R.C., Candiotti K.A., Lubarsky D.A, & Hao S. (2016). HSV vector-mediated GAD67 suppresses neuropathic pain induced by perineural HIV gp120 in rats through inhibition of ROS and Wnt5a. Gene therapy, 23(4), 340-348.
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2

Immunohistochemical Mapping of GABA in Spinal Cord

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The distribution of GABA in the SDH was determined by immunohistochemistry. Rats were perfused intracardially with 4% paraformaldehyde in 0.1 M phosphate buffer, and the L4–L5 segments of the spinal cord were removed, postfixed in the same solution for overnight, and cryoprotected with 30% sucrose in PBS for 2 days. Cryostat sections (35µm thickness) were incubated overnight at 4 °C with rabbit anti-GABA (1:1000; a gift from Dr. Yuan Zhu, Department of Medicine, University of Michigan, MI), mouse anti-GFAP antibody (1 : 2000, cata# G3893, Sigma, St. Louis, MO), mouse anti-OX42 antibody (1:1000, cata# CBL1512, Millipore, Billerica, MA), mouse anti-NeuN monoclonal antibody (A60) (1 : 5000, cata# MAB377, Millipore, Billerica, MA), or rabbit anti-Wnt5a (1:1000, cata# ab72583, Abcam, Cambridge, MA) followed by fluorescent IgG (Alexa Fluor 488, 1:1000 or Alexa Fluor 594, Molecular Probes, Eugene, OR, USA) for 2 hours at room temperature. Fluorescence images were captured by a fluorescent microscopy (Fluorescent M Leica/Micro CDMI 6000B)29 (link), 88 (link).
Kanda H., Kanao M., Liu S., Yi H., Iida T., Levitt R.C., Candiotti K.A., Lubarsky D.A, & Hao S. (2016). HSV vector-mediated GAD67 suppresses neuropathic pain induced by perineural HIV gp120 in rats through inhibition of ROS and Wnt5a. Gene therapy, 23(4), 340-348.
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