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Dil and bodipy tr ceramide 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt tr dhpe

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Dil and BODIPY-TR-ceramide, 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE) are fluorescent labeling reagents used in various biological applications. Dil is a lipophilic dye, and BODIPY-TR-ceramide is a fluorescent ceramide analog. TR-DHPE is a fluorescent phospholipid. These products can be used for cell membrane labeling, trafficking studies, and other fluorescence-based techniques.

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2 protocols using dil and bodipy tr ceramide 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt tr dhpe

1

Lipid Membrane Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
BODIPY-sphingosine was a gift from Robert Bittman (Queens College of CUNY Flushing, NY). Reagents purchased from commercial sources: methyl-β-cyclodextrin, cholesterol-methyl-β-cyclodextrin (cholesterol-water soluble), 2-hydroxypropyl-β-cyclodextrin and sphingomyelinase from Bacillus cereus (Sigma-Aldrich, St. Louis, MO); Dil and BODIPY-TR-ceramide, 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE)(Life Technologies, Carlsbad, CA); TopFluor PS, brain sphingosine, egg PC, liver PE, cholesterol from sheep wool, brain PS, egg PA, brain PI(4)P, brain PI(4,5)P2, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (Avanti Polar Lipids, Alabaster, AL); and ATP, [γ-32P] (PerkinElmer, Waltham, MA). Electron microscopy reagents were purchased from Electron Microscopy Sciences.
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2

Lipid Membrane Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
BODIPY-sphingosine was a gift from Robert Bittman (Queens College of CUNY Flushing, NY). Reagents purchased from commercial sources: methyl-β-cyclodextrin, cholesterol-methyl-β-cyclodextrin (cholesterol-water soluble), 2-hydroxypropyl-β-cyclodextrin and sphingomyelinase from Bacillus cereus (Sigma-Aldrich, St. Louis, MO); Dil and BODIPY-TR-ceramide, 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE)(Life Technologies, Carlsbad, CA); TopFluor PS, brain sphingosine, egg PC, liver PE, cholesterol from sheep wool, brain PS, egg PA, brain PI(4)P, brain PI(4,5)P2, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (Avanti Polar Lipids, Alabaster, AL); and ATP, [γ-32P] (PerkinElmer, Waltham, MA). Electron microscopy reagents were purchased from Electron Microscopy Sciences.
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