Hplc grade ammonium formate

Manufactured by Merck Group

Sourced in United States, Germany

HPLC-grade ammonium formate is a high-performance liquid chromatography (HPLC) reagent used as a mobile phase additive. It is a salt composed of ammonium cations and formate anions, providing a pH-modifying agent for HPLC applications.

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Lab products found in correlation

Hplc grade formic acid, by Merck Group (8 mentions) Formic acid, by Merck Group (6 mentions) Ms grade acetonitrile, by Thermo Fisher Scientific (6 mentions) Hplc grade 2 propanol, by Thermo Fisher Scientific (6 mentions) Ms grade methanol, by Thermo Fisher Scientific (6 mentions) Acetonitrile, by Thermo Fisher Scientific (5 mentions) Acetonitrile, by Merck Group (3 mentions) Methanol, by Merck Group (3 mentions) Methanol, by Avantor (2 mentions) Ammonium acetate, by Merck Group (2 mentions) Methyl tert butyl ether (mtbe), by Merck Group (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Acetic acid, by Merck Group (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Ethyl acetate, by Merck Group (2 mentions) Glycocholic acid, by Merck Group (1 mentions) Glycodeoxycholic acid, by Merck Group (1 mentions) Deoxycholic acid, by Merck Group (1 mentions) Ursodeoxycholic acid, by Merck Group (1 mentions) Glycoursodeoxycholic acid, by Merck Group (1 mentions)

Close spelling variants of this product

Ammonium formate (856 citations) HPLC grade (319 citations)

20 protocols using hplc grade ammonium formate

Analytical Extraction and Quantification of Broflanilide, DM-8007, and S(PFH-OH)-8007

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Broflanilide (99.67% purity), DM-8007 (99.86% purity), and S(PFH-OH)-8007 (99.02% purity) were obtained from the Dongbang Agro Corporation (Korea). LC-gradient-grade acetonitrile for sample extraction and methanol were purchased from J. T. Baker (USA). HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA), respectively. The QuEChERS extraction pouch for sample extraction and the d-SPE tube for sample purification were purchased from Agilent Technologies (USA). A Combi-514R centrifuge (Hanil Scientific Inc., Korea) and a 1600 MiniG extractor (SPEX SamplePrep, USA) were used during sample preparation.

Noh H.H., Kim C.J., Kwon H., Kim D., Moon B.C., Baek S., Oh M.S, & Kyung K.S. (2020). Optimized residue analysis method for broflanilide and its metabolites in agricultural produce using the QuEChERS method and LC-MS/MS. PLoS ONE, 15(10), e0235526.

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Quantification of Bile Acids in Biological Samples

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Cholic acid (CA), chenodeoxyCholic acid (CDCA), ursodeoxyCholic acid (UDCA), deoxyCholic acid (DCA), lithoCholic acid (LCA), tauroCholic acid (TCA), taurochenodeoxyCholic acid (TCDCA), tauroursodeoxyCholic acid (TUDCA), taurodeoxyCholic acid (TDCA), taurolithoCholic acid (TLCA), glycoCholic acid (GCA), glycochenodeoxyCholic acid (GCDCA), glycoursodeoxyCholic acid (GUDCA), glycodeoxyCholic acid (GDCA), and lithoCholic acid-2,2,3,4,4-d5 (internal standard) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). GlycolithoCholic acid (GLCA) was purchased from J&K Scientific Ltd. (Shanghai, China). HPLC-grade ammonium formate (≥99%), ammonium acetate, methanol, and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). HPLC-grade formic acid (99%) was purchased from Anaqua Chemicals Supply (Wilmington, USA).

Qu Y., Su C., Zhao Q., Shi A., Zhao F., Tang L., Xu D., Xiang Z., Wang Y., Wang Y., Pan J, & Yu Y. (2022). Gut Microbiota-Mediated Elevated Production of Secondary Bile Acids in Chronic Unpredictable Mild Stress. Frontiers in Pharmacology, 13, 837543.

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Analytical Procedure for Agrochemical Compounds

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Broflanilide (99.67% purity), DM-8007 (99.86% purity), and S(PFH-OH)-8007 (99.02% purity) were obtained from the Dongbang Agro Corporation (Korea). LC-gradientgrade acetonitrile for sample extraction and methanol were purchased from J. T. Baker (USA). HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA),

Noh H.H., Kim C.J., Kwon H., Kim D., Moon B., Baek S., Velicanu D., & Kyung K.S. (2020). Optimized residue analysis method for broflanilide and its metabolites in agricultural produce using the QuEChERS method and LC-MS/MS.

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Comprehensive Herbal Combination Characterization

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MS-grade methanol, MS-grade acetonitrile, HPLC-grade 2-propanol were purchased from Thermo Fisher. HPLC-grade formic acid and HPLC-grade ammonium formate were purchased from Sigma. CSS is composed of seven herbs, including Citrus reticulata Blanco, Radix Bupleuri, Ligusticum chuanxiong Hort, CyperusrotundusL., Citrus aurantium L., Paeonia lactiflora Pall. and Glycyrrhiza uralensis Fisch., and the ratio is 4:4:3:3:3:3:1. The herbs were purchased from the pharmacy of Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine. All herbs were authenticated by the herbal medicine botanist Professor Yunan, Zhao, Nanjing University of Chinese Medicine.

Li W., Zhou R., Zheng J., Sun B., Jin X., Hong M, & Chen R. (2022). Chaihu-Shugan-San ameliorates tumor growth in prostate cancer promoted by depression via modulating sphingolipid and glycerinphospholipid metabolism. Frontiers in Pharmacology, 13, 1011450.

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Procurement of MS-grade Solvents

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MS-grade methanol, MS-grade acetonitrile and HPLC-grade isopropanol were purchased from ThermoFisher (Thermo Fisher Scientific Co., Waltham, Massachusetts, USA). HPLC-grade formic acid, HPLC-grade ammonium formate and MTBE were purchased from Sigma-Aldrich (Sigma Chemical Co., St. Louis, Missouri, USA).

Ban Y., Ran H., Chen Y, & Ma L. (2021). Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study. Journal of Ovarian Research, 14, 135.

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Metabolite Quantification Using LC-MS

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Sigma Aldrich provided Ammonium acetate (NH₄AC), Merck provided Acetonitrile, and Fisher provided ammonium hydroxide (NH₄OH) and methanol. Thermo Fisher provided MS-grade methanol, MS-grade Acetonitrile, and HPLC-grade 2-propanol. Sigma provided HPLC-grade formic acid and HPLC-grade ammonium formate. All chemicals used in this study were of high purity and quality, ensuring the reliability and reproducibility of the results.

Qiao C., He M., Wang S., Jiang X., Wang F., Li X., Tan S., Chao Z., Xin W., Gao S., Yuan J., Li Q., Xu Z., Zheng X., Zhao J, & Liu G. (2023). Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs. Frontiers in Microbiology, 14, 1250891.

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Tanshinone Compound Quantification Method

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MS-grade methanol, MS-grade acetonitrile and high-performance liquid chromatography (HPLC)-grade 2-propanol were purchased from Thermo Fisher. HPLC-grade formic acid and HPLC-grade ammonium formate were purchased from Sigma. HPLC-grade acetonitrile was purchased from Merck KGaA (Darmstadt, Germany). Analytical-grade pure phosphoric acid was purchased from Guangzhou Chemical Reagent Factory. Reference standards of Tanshinone I, Crytotanshinone, Tanshinone IIA (purity >98%) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Diydrotanshinone I (purity >98%) was purchased from Guangzhou Qiyun Biotechnology Co., Ltd. (Guangdong, China).

Chen T., Zhu Z., Du Q., Wang Z., Wu W., Xue Y., Wang Y., Wu Y., Zeng Q., Jiang C., Shen C., Liu L., Zhu H, & Liu Q. (2021). A Skin Lipidomics Study Reveals the Therapeutic Effects of Tanshinones in a Rat Model of Acne. Frontiers in Pharmacology, 12, 675659.

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Mass Spectrometry Lipid Analysis

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LC–MS grade water (H₂O) and methanol (MeOH) were from VWR International (Plainview, NY). HPLC grade ammonium formate, chloroform, 2-propanol (IPA) and formic acid were from Sigma-Aldrich (Sigma Chemical Co., St Louis, MO, USA). All internal standards used in this study (see Table S1 of the supplemental data) were purchased from Avanti Polar Lipids, Inc (Alabaster, AL).

Seyer A., Boudah S., Broudin S., Junot C, & Colsch B. (2016). Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow. Metabolomics, 12, 91.

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Acquisition of Mass Spectrometry Reagents

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Mass Spec grade methanol, acetonitrile and high-performance liquid chromatography grade (HPLC-grade) 2-propanol were purchased from Thermo Fisher Scientific (Waltham, MA). HPLC-grade formic acid and HPLC-grade ammonium formate were purchased from Sigma-Aldrich (St. Louis, MO).

He Q., Guo X., Lv W., Cui J., Meng J., Gao X., Ma J., Zhou N, & Cao Y. (2024). Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome. Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology, 44(1).

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Analytical Reagent Procurement Protocol

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MS-grade methanol, MS-grade acetonitrile, HPLC-grade isopropanol were purchased from ThermoFisher (Thermo Fisher Scienti c Co., Waltham, Massachusetts, USA). HPLC-grade formic acid, HPLC-grade ammonium formate and MTBE were purchased from Sigma-Aldrich (Sigma Chemical Co., St. Louis, Missouri, USA).

Ban Y., Ran H., Chen Y., & Ma L. (2021). Lipidomics Analysis of Human Follicular Fluid Form Normal-weight Patients With Polycystic Ovary Syndrome.

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