Rnase free ddh2o

FDAA probes were purchased from the Chinese Peptide Company. The REPLI‐g Mini Kit was purchased from Qiagen. Lysis buffer for direct PCR of microorganisms was purchased from Takara Biomedical Technology. The 2× Taq PCR mix, RNase‐free ddH₂O, and phosphate‐buffered saline (PBS) were purchased from Sangon Biotech.

All oligonucleotides (HPLC purified) were synthesized and modified by Sangon Biotechnology Co., Ltd. (Shanghai, China). D-PBS buffer, 20× PBS buffer, 5× TBE buffer, RNase-free ddH₂O, Tween-20, Bovine Serum Albumin (BSA), HRP-labeled Streptavidin (SA-HRP), and EL-TMB Chromogenic Reagent kit were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). ASFV p30 recombinant protein, p30 monoclonal antibody, porcine reproductive and respiratory syndrome virus (PRRSV) recombinant N protein, staphylococcus aureus enterotoxin type A recombinant protein (SEA), and pig immunoglobulin G (IgG) were purchased from Aiyi Biotechnology. (Nanjing, China). 6× His tag peptides were bought from Synpeptide Biotechnology Co., Ltd. (Nanjing, China). Swine serum was sourced from Solarbio Science & Technology Co., Ltd. (Beijing, China). NHS (N-hydroxysuccinimide)-Activated Magnetic Beads (NHS-MB) were purchased from Beaver Biosciences Inc. (Suzhou, China). Taq PCR Master Mix and ChamQ Universal SYBR qPCR Master Mix were purchased from Vazyme Biotechnology Co., Ltd. (Nanjing, China). The 96-well polystyrene microtiter plate (MaxiSorp) was bought from Thermo Fisher Scientific (Shanghai, China). ASFV-positive freeze-dried serum, porcine negative serum national reference was purchased from the National Center of Veterinary Culture collection (CVCC) (Beijing, China).