Fluconazole

Perillaldehyde (CAS registry no. 18031-40-8) was obtained from the TCI Development Co., Ltd. (Tokyo, Japan), prepared as a stock solution in 0.1% (v/v) Tween-80. Fluconazole and estradiol benzoate oil were acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China).

All the antimicrobial peptides were purchased from GL Biochem (Shanghai) Ltd. (Shanghai, China) and their purity was determined to be higher than 98% using reversed-phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Mueller Hinton Broth (MHB), Mueller Hinton Agar (MHA), Potato Dextrose Broth (PDB), Potato Dextrose Agar (PDA), clindamycin hydrochloride (CDHC), and fluconazole were purchased from Solarbio Life Science (Beijing, China). CCK-8 kit was obtained from Dojindo Molecular Technologies, Inc. (Tokyo, Japan). Urea assay kit, alanine aminotransferase assay kit, aspartate aminotransferase assay kit and creatinine assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Acetonitrile (ACN) and trifluoroacetic acid (TFA) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SYPRO Orange protein gel stain was purchased from Sigma-Aldrich (St. Louis, MO, USA). All the reagents were of commercial special grade and were used without further purification.