ChIP experiments were performed as previously described in33 (link) with minor modifications. Briefly, S.pombe cells were fixed with 1% formaldehyde for 15 min and then lysed in buffer containing 50 mM HEPES/KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na deoxycholate, 1 mM PMSF and protease-inhibitor cocktail. Chromatin was sheared with a Bioruptor (Diagenode). The following antibodies were used in this study: histone H3K9me2-specific mouse monoclonal antibody from Wako (clone no. MABI0307), histone H3-specific rabbit polyclonal antibody from Abcam (clone no. ab1791), histone H3K36me3-specific rabbit polyclonal antibody from Abcam (clone no. ab9050), and RNA Polymerase II mouse monoclonal antibody from Covance (clone no. 8WG16).
Histone h3k9me2 specific mouse monoclonal antibody
Manufactured by Fujifilm
The Histone H3K9me2-specific mouse monoclonal antibody is a laboratory reagent used for the detection and analysis of histone H3 proteins with dimethylation at lysine 9. This antibody can be utilized in various biochemical and molecular biology techniques, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation.
Automatically generated - may contain errors
3 protocols using histone h3k9me2 specific mouse monoclonal antibody
1
ChIP Experiments in S. pombe
2
ChIP Experiments in S. pombe
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ChIP experiments were performed as previously described in33 (link) with minor modifications. Briefly, S.pombe cells were fixed with 1% formaldehyde for 15 min and then lysed in buffer containing 50 mM HEPES/KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na deoxycholate, 1 mM PMSF and protease-inhibitor cocktail. Chromatin was sheared with a Bioruptor (Diagenode). The following antibodies were used in this study: histone H3K9me2-specific mouse monoclonal antibody from Wako (clone no. MABI0307), histone H3-specific rabbit polyclonal antibody from Abcam (clone no. ab1791), histone H3K36me3-specific rabbit polyclonal antibody from Abcam (clone no. ab9050), and RNA Polymerase II mouse monoclonal antibody from Covance (clone no. 8WG16).
3
Histone H3K9me2 ChIP Protocol
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ChIP experiments were performed as described previously [18 (link)] with a histone H3K9me2-specific mouse monoclonal antibody from Wako (clone no. MABI0307).
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