Yttrium spa beads

Manufactured by PerkinElmer

Yttrium SPA beads are a type of scintillation proximity assay (SPA) beads made from yttrium oxide. SPA beads are designed to facilitate the detection and measurement of radioactive signals within a biological sample. The yttrium oxide composition of these beads provides a high-density substrate that efficiently captures and amplifies the radioactive signal, enabling sensitive and accurate assay results.

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Lab products found in correlation

Cytodynamix screen15, by Cytoskeleton (4 mentions) Colchicine, by PerkinElmer (4 mentions) 3h colchicine, by PerkinElmer (4 mentions) Colchicine, by Cytoskeleton (1 mentions)

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SPA beads (7 citations)

4 protocols using yttrium spa beads

Colchicine Site Competitive Assay

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Example 6

The affinity of compounds 8c to colchicine binding site was determined using colchicine Site Competitive Assay kit CytoDYNAMIX Screen15 (Cytoskeleton, Inc., CO, USA) using the standard protocol of the manufacturer to determine Ki values (μM). Biotin-labelled tubulin (0.5 g) in 10 μL of reaction buffer was mixed with [3H]colchicine (0.08 μM, PerkinElmer, Waltham, Mass.) and the test compounds (positive control colchicine, negative control vinblastine, G-1, fluorescent G-1, or 2-ME) in a 96-well plate (final volume: 100 μL). After incubating for 2 h at 37° C. with gentle shaking, streptavidin-labelled yttrium SPA beads (80 g in 20 μL reaction buffer, PerkinElmer, Waltham, Mass.) were added to each well and incubated for 30 min at 4° C. The plates were then read on a scintillation counter (Packard Instrument, Topcount Microplate Reader) and the percentage of inhibition was calculated [54]

US10882852B1. 3-vinylquinolines as cancer cells inhibitors (2021-01-05). King Abdulaziz University [SA]. Inventors: Tarek Salah Ibrahim [SA], Mohamed Moustafa Hawwas [EG], Azizah M. Malebari [SA], Moustafa E. El-Araby [SA], Abdelsattar M. Omar [SA].

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Benzotriazole Compounds Colchicine Site Assay

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The affinity of Benzotriazole compounds to colchicine binding site was determined using a colchicine Site Competitive Assay kit CytoDYNAMIX Screen15 (Cytoskeleton, Inc., Denver, CO, USA) using the standard protocol of the manufacturer to determine Ki values (μM). Biotin-labelled tubulin (0.5 μg) in 10 μL of reaction buffer was mixed with [3H]colchicine (0.08 μM, PerkinElmer, Waltham, MA) and the test compounds (positive control colchicine, negative control vinblastine, G-1, fluorescent G-1, or 2-ME) in a 96-well plate (final volume: 100 μL). After incubating for 2 h at 37 °C with gentle shaking, streptavidin-labelled yttrium SPA beads (80 μg in 20 μL reaction buffer, PerkinElmer, Waltham, MA) were added to each well and incubated for 30 min at 4 °C. The plates were then read on a scintillation counter (Packard Instrument, Topcount Microplate Reader) and the percentage of inhibition was calculated [55 (link),56 (link)].

Khayyat A.N., Mohamed K.O., Malebari A.M, & El-Malah A. (2021). Design, Synthesis, and Antipoliferative Activities of Novel Substituted Imidazole-Thione Linked Benzotriazole Derivatives. Molecules, 26(19), 5983.

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Colchicine Site Competitive Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 6

The affinity of compounds 8c to colchicine binding site was determined using colchicine Site Competitive Assay kit CytoDYNAMIX Screen15 (Cytoskeleton, Inc., CO, USA) using the standard protocol of the manufacturer to determine Ki values (μM). Biotin-labelled tubulin (0.5 μg) in 10 μL of reaction buffer was mixed with [3H]colchicine (0.08 μM, PerkinElmer, Waltham, Mass.) and the test compounds (positive control colchicine, negative control vinblastine, G-1, fluorescent G-1, or 2-ME) in a 96-well plate (final volume: 100 μL). After incubating for 2 h at 37° C. with gentle shaking, streptavidin-labelled yttrium SPA beads (80 μg in 20 μL reaction buffer, PerkinElmer, Waltham, Mass.) were added to each well and incubated for 30 min at 4° C. The plates were then read on a scintillation counter (Packard Instrument, Topcount Microplate Reader) and the percentage of inhibition was calculated [54]

US11345692B1. 3-vinylquinolines as cancer cells inhibitors (2022-05-31). King Abdulaziz University [SA]. Inventors: Tarek Salah Ibrahim [SA], Mohamed Moustafa Hawwas [EG], Azizah M. Malebari [SA], Moustafa E. El-Araby [SA], Abdelsattar M. Omar [SA].

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Colchicine Site Binding Assay of Compound 12c

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The affinity of compounds 12c to colchicine binding site was determined using colchicine Site Competitive Assay kit CytoDYNAMIX Screen15 (Cytoskeleton, Inc., Denver, CO, USA) using the standard protocol of the manufacturer to determine Ki. Biotin-labelled tubulin (0.5 µg) in 10 µL of reaction buffer was mixed with [3H]colchicine (0.08 µM, PerkinElmer, Waltham, MA) and the test compounds (positive control colchicine, negative control vinblastine, G-1, fluorescent G-1, or 2-ME) in a 96-well plate (final volume: 100 µL). After incubating for 2 h at 37 °C with gentle shaking, streptavidin-labelled yttrium SPA beads (80 µg in 20 µL reaction buffer, PerkinElmer, Waltham, MA) were added to each well and incubated for 30 min at 4 °C. The plates were then read on a scintillation counter (Packard Instrument, Topcount Microplate Reader) and the percentage of inhibition was calculated⁵⁰ (link)^,⁵¹ (link).

Ibrahim T.S., Hawwas M.M., Malebari A.M., Taher E.S., Omar A.M., Neamatallah T., Abdel-Samii Z.K., Safo M.K, & Elshaier Y.A. (2021). Discovery of novel quinoline-based analogues of combretastatin A-4 as tubulin polymerisation inhibitors with apoptosis inducing activity and potent anticancer effect. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1), 802-818.

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