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Girk2

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GIRK2 is a G protein-coupled inwardly-rectifying potassium channel protein. It plays a role in regulating neuronal excitability and is involved in the modulation of synaptic transmission.

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Lab products found in correlation

Foxa2, by Abcam (3 mentions) Foxg1, by Abcam (3 mentions) Olig2, by Merck Group (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Welgene (2 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Lrtm1, by Cusabio (2 mentions) Psd 95, by Thermo Fisher Scientific (2 mentions) Triton x 100, by Merck Group (2 mentions) Nurr1, by Santa Cruz Biotechnology (2 mentions) Foxa2, by Santa Cruz Biotechnology (1 mentions) Digital sight ds u1, by Nikon (1 mentions) Nestin, by Abcam (1 mentions) Vmat2, by Merck Group (1 mentions) Lmx1a, by Merck Group (1 mentions) Ps129 α synuclein, by Abcam (1 mentions) Parkin, by Merck Group (1 mentions) Vmat 2, by Abcam (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Shandon cryotome, by Thermo Fisher Scientific (1 mentions) Mash1, by BD (1 mentions)

4 protocols using girk2

1

Multiparametric Immunohistochemical Profiling

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The dried tissue sections were permeabilized and blocked with DPBS (Welgene) containing 0.03% Triton X-100 (Sigma) and 5% FBS (Seradigm) for 1 hr at room temperature. The permeabilized tissue sections were then incubated with the appropriate primary antibody for 16 hr at 4°C, and then incubated with the appropriate secondary antibody after being washed three times with DPBS (Welgene). Counterstaining was performed with TOPRO-3 (Life Technology). Primary antibodies used for immunohistochemistry are as follows: MAP2 (Sigma, 1 : 500), TH (Abcam, 1 : 500), TH (R&D system, 1 : 500), TBR1 (Abcam, 1 : 500), TUJ1 (Biolegend, 1 : 1,000), SOX2 (R&D system, 1 : 500), FOXA2 (Abcam, 1 : 500), LRTM1 (Cusabio, 1 : 500), GIRK2 (Abcam, 1 : 500), FOXG1 (Abcam, 1 : 500), GABA (Sigma, 1 : 500), GLUT (Cusabio, 1 : 500), GFAP (CiteAb, 1 : 200), S100β (Abcam, 1 : 200), MBP (Abcam, 1 : 200), AQP4 (Abcam, 1 : 500), O4 (R&D system, 1 : 500), OLIG2 (Merck Millipore, 1 : 500), PSD-95 (Thermo Fisher, 1 : 300), SYP (Cusabio, 1 : 500), Dopamine (ImmuSmol SAS, 1 : 500), and caspase 3 (Cell Signaling, 1 : 500).
Yao X., Kang J.H., Kim K.P., Shin H., Jin Z.L., Guo H., Xu Y.N., Li Y.H., Hali S., Kwon J., La H., Park C., Kim Y.J., Wang L., Hong K., Cao Q., Cho I.J., Kim N.H, & Han D.W. (2023). Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells. Stem Cells International, 2023, 3320211.
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2

Multiparametric Immunohistochemical Profiling

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The dried tissue sections were permeabilized and blocked with DPBS (Welgene) containing 0.03% Triton X-100 (Sigma) and 5% FBS (Seradigm) for 1 hr at room temperature. The permeabilized tissue sections were then incubated with the appropriate primary antibody for 16 hr at 4°C, and then incubated with the appropriate secondary antibody after being washed three times with DPBS (Welgene). Counterstaining was performed with TOPRO-3 (Life Technology). Primary antibodies used for immunohistochemistry are as follows: MAP2 (Sigma, 1 : 500), TH (Abcam, 1 : 500), TH (R&D system, 1 : 500), TBR1 (Abcam, 1 : 500), TUJ1 (Biolegend, 1 : 1,000), SOX2 (R&D system, 1 : 500), FOXA2 (Abcam, 1 : 500), LRTM1 (Cusabio, 1 : 500), GIRK2 (Abcam, 1 : 500), FOXG1 (Abcam, 1 : 500), GABA (Sigma, 1 : 500), GLUT (Cusabio, 1 : 500), GFAP (CiteAb, 1 : 200), S100β (Abcam, 1 : 200), MBP (Abcam, 1 : 200), AQP4 (Abcam, 1 : 500), O4 (R&D system, 1 : 500), OLIG2 (Merck Millipore, 1 : 500), PSD-95 (Thermo Fisher, 1 : 300), SYP (Cusabio, 1 : 500), Dopamine (ImmuSmol SAS, 1 : 500), and caspase 3 (Cell Signaling, 1 : 500).
Yao X., Kang J.H., Kim K.P., Shin H., Jin Z.L., Guo H., Xu Y.N., Li Y.H., Hali S., Kwon J., La H., Park C., Kim Y.J., Wang L., Hong K., Cao Q., Cho I.J., Kim N.H, & Han D.W. (2023). Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells. Stem Cells International, 2023, 3320211.
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3

Immunofluorescence Characterization of Neuronal Cells

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The following primary antibodies were used: AADC (Millipore/AB1569), ASCL1 (Millipore/AB5696), DAT (Abcam/AB5990), FOXA2 (Santa Cruz/SC-6554), FOXG1 (Abcam/AB18259), GIRK2 (Abcam/AB30738), KI67 (DAKO/M7240), LMX1A (Millipore/AB10533), NESTIN (Abcam/AB22035), NGN2 (R&D Systems/MAB3314), NURR1 (Santa Cruz/SC-990), SOX1 (Millipore/AB15766), SOX2 (R&D Systems/MAB2018), TH (Millipore/MAB5280), TH (Millipore/AB152), TUJ1 (Biolegend/801202), VMAT2 (Millipore/AB1598P). Fluorescent imaging was performed on a Nikon Eclipse TE2000U microscope with an Optronics Microfire camera or Photometrics Evolve EMCCD camera. Phase contrast images were taken on the Nikon Eclipse TS100 microscope with a Nikon Digital Sight DS-U1 camera. For cell quantification experiments, the investigator was blinded to the condition during image acquisition and counting.
Playne R., Jones K, & Connor B. (2018). Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors. Journal of Stem Cells & Regenerative Medicine, 14(1), 34-44.
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4

Immunohistochemical Analysis of Organoid Sections

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Organoids were washed with 1× PBS before being fixed in 4% paraformaldehyde in PBS and cryoprotected in a 30% sucrose solution overnight. Frozen organoids were sectioned at 15–20 μm using a Thermo Shandon cryotome and collected on glass microscope slides. Sections were immunostained according to standard protocols using the following primary antibodies: OTX2 (Abcam, AB21990), FOXA2 (Abcam, AB108422), NGN2 (Millipore, AB5682), MASH1 (BD Biosciences, 556604), TUJ1 (Sigma, T2200), MAP2 (Cell Signaling Technologies, 4542s), TH (Pel-Freez Biologicals, P60101-150), VMAT2 (Abcam, AB1598P), DAT (Millipore, MAB369), PITX3 (Invitrogen, 382850), AADC (Abcam, AB211535), NURR1 (Santa Cruz, sc-991), GIRK2 (Abcam, AB65096), cleaved caspase-3 (Cell Signaling, 9661s), pS129-α-synuclein (Abcam, AB9850), LAMP1 (Developmental Studies Hybridoma Bank), γH2AX (Cell Signaling, 9718s), LC3B (Cell Signaling, 3868s), PARKIN (Millipore, MAB5512), NRF2 (Cell Signaling, 12721s), and EEA1 (Millipore, 07-1820). Appropriate fluorescent secondary antibodies were obtained from Invitrogen. Next, sections were treated with 6-diamidino-2-phenylindole (Invitrogen) and mounted in Fluoromount-G mounting medium. Representative images were captured using a Nikon Eclipse Ti microscope and a confocal laser scanning microscope (Zeiss, LSM800).
Kim H., Park H.J., Choi H., Chang Y., Park H., Shin J., Kim J., Lengner C.J., Lee Y.K, & Kim J. (2019). Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem Cell Reports, 12(3), 518-531.
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