Tissue tek container

The biopsy samples were placed in a tissue tek container (Sakura Finetek, CA, USA) and then filled with tissue tek OCT compound gel. After being cut into 7-µm slices, the samples were snap-frozen in liquid nitrogen and stained with Oil Red O according to standard procedures.
The oil red O fat staining method is usually used to detect fat in tissues or cells. Oil red O is a fat-soluble dye that is a strong fat solvent and fat dye and can be highly dissolved in fat. Its dyeing principle is that oil red O can specifically adsorb with the neutral triglycerides, lipids and lipoproteins in tissues and cells to make fat dye. The solubility of dye in intracellular lipids is greater than that in solution.

The biopsy samples were placed in a tissue tek container (Sakura Finetek, CA, USA) and then filled with tissue tek OCT compound gel. After being cut into 7-μm slices, the samples were snap-frozen in liquid nitrogen and stained with Oil Red O according to standard procedures.
The oil red O ²⁴ fat staining method is usually used to detect the fat in tissues or cells. Oil red O is a fat-soluble dye, which is a strong fat solvent and fat dye, and can be highly dissolved in fat. Its dyeing principle is that oil red O can specifically adsorb with the neutral triglycerides, lipids and lipoproteins in tissues and cells to make fat dye. The solubility of dye in intracellular lipids is greater than that of solution.

Atrial tissues of MR patients and aortic valve disease patients were sampled from the left atrial free wall during surgery. After excision, some atrial tissues were immediately frozen in liquid nitrogen. Additionally, some atrial tissues were placed into a Tissue Tek^® container which was then filled with Tissue Tek^® optimum cutting temperature compound (Sakura^® Finetek, CA, USA) and these samples were frozen in liquid nitrogen for later histochemical study.