Originpro 2015 sr2

Collected data were analyzed and plotted using Prism 5 Software (GraphPad Software Inc). As Gaussian normality was rejected by the Kolmogorov–Smirnov test for at least one group per experiment, nonparametric statistics was used for analysis. The Kruskal–Wallis test was applied to check for differences between multiple groups and the Mann–Whitney U test for pairwise comparisons. Data are represented as box plots with whiskers representing the 10th to 90th percentiles. Outliers are not depicted, but the mean is represented as a “+”. A p-value of p ≥ 0.05 was regarded as not significant (n.s.), p ≤ 0.05 as a trend (∗), p ≤ 0.01 as significant (∗∗), and p ≤ 0.001 as highly significant (∗∗∗). Plots of individual cell tracks were generated using OriginPro 2015 SR2 software (OriginLab Corporation). Stacked bar diagrams were generated using Excel (Microscoft).

A one-way analysis of variance test was performed for comparison of different data groups, followed by Tukey’s post hoc test. Normality assumption and equal variance were verified by setting the P-values to 0.05, 0.01 or 0.001 using the Shapiro–Wilk’s and Levene Median tests, respectively (OriginPro 2015 Sr2, OriginLab Corp., USA).

Data are displayed as box-and-whisker plots. The boxes are 25th−75th quartiles, whiskers range from 10th to 90th percentiles, the mean is marked as a square and the median as a line. Bar graphs show mean±s.e.m. values. Using OriginPro 2015 SR2 (OriginLab Corporation), paired two-tailed Student's t-test was used to calculate the statistical significance against control values, one-way ANOVA with post-hoc Bonferroni test and Mann–Whitney U-test were performed for multiple and pairwise comparisons. Significance values are indicated as *P≤0.05; **P≤0.01; ***P≤0.001 and ****P≤0.0001.