Freestyle optimum glucometer

Height and weight were obtained using standardised techniques and instruments. The body mass index (BMI) was calculated as the weight in kilograms divided by the square of the height in meters [weight (kg)/Height (m²)]. Fasting blood glucose levels were measured by the FreeStyle optimum glucometer (Abbott Diabetes Care, Australia). HbA1c levels were measured by the Bayer A1C Now⁺ Multi test A1C system (Catalog no.08842610). Creatinine (Siemens #DF33A) and uric acid (Siemens #BA4007) were measured by Siemens automated analyser (Dimension Xpand ^plus), estimated Glomerular Filtration Rate (eGFR) calculated from creatinine by using Modification of Diet in Renal Disease (MDRD) formula. Serum Insulin and C-peptide levels were measured by Bio-Rad Multiplex assay kit (Catalog:#171-A7001M). Assay was performed as per the manufacturer instructions.

Height and weight were obtained using standardised techniques and instruments. The body mass index (BMI) was calculated as the weight in kilograms divided by the square of the height in meters [weight (kg)/Height (m²)]. Fasting blood glucose levels were measured by the Free Style optimum glucometer (Abbott Diabetes Care, Australia). HbA1c levels were measured by the Bayer A1C Now⁺ Multi test A1C system (Catalog no.08842610). Creatinine, uric acid, total cholesterol, triglycerides, high density lipoprotein (HDL) cholesterol, and CK-MB levels were measured by Siemens automated analyser (Dimension Xpand^plus). Estimated glomerular filtration rate (eGFR) calculated from creatinine by using Modification of Diet in Renal Disease (MDRD) formula. Low density lipoprotein (LDL) cholesterol concentrations were calculated by using the Friedewald formula. ApoB (mg/dl) was calculated based on the formula ApoB  =  −33.12  +  0.675*LDL  +  11.95*ln (Triglycerides)34 (link).