Dulbecco s modified eagle s cell culture media dmem

Manufactured by Thermo Fisher Scientific

Sourced in United States

Dulbecco's modified Eagle's cell culture media (DMEM) is a widely used cell culture medium formulated to support the growth and maintenance of a variety of cell types in vitro. It provides a balanced salt solution, amino acids, vitamins, and other essential nutrients required for cell proliferation.

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Lab products found in correlation

Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Thermo Fisher Scientific (2 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Atp na2, by Merck Group (1 mentions)

2 protocols using dulbecco s modified eagle s cell culture media dmem

HPLC Analysis of Cell Culture Media

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High-performance liquid chromatography (HPLC) grade methanol and acetonitrile were purchased from Fisher Scientific (Waltham, USA). Internal standard l-methionine sulfone and testosterone propionate were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. Dulbecco’s modified Eagle’s cell culture media (DMEM), Roswell Park Memorial Institute (RPMI) 1640 media, fetal calf serum (FCS) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were all obtained from Gibco-BRL (Grand Island, New York, USA).

Wang H., Hu J.H., Liu C.C., Liu M., Liu Z, & Sun L.X. (2018). LC-MS based cell metabolic profiling of tumor cells: a new predictive method for research on the mechanism of action of anticancer candidates. RSC Advances, 8(30), 16645-16656.

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Evaluation of Sodium-dependent Biomolecules

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All standards (UTP-Na, ADP-Na₂, AMP-Na₂, ATP-Na₂, CTP-Na₂, GDP-Na₂, GMP-Na₂, UDP-Na₂, dUTP-Na₃, dATP-Na₃, dCTP-Na₃, dGTP-Na₄) were purchased from Sigma (St. Louis, USA). Tetrabutylammonium hydrogen sulfate (TBAHS) was obtained from Tianjin Kermel Chemical Reagent Co., Ltd. Methanol (HPLC grade) was obtained from Shandong Yuwang Industrial Co., Ltd. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypsin were provided by Amresco LLC (Solon, USA). All other chemical reagents used, analytical grade, were purchased from Tianjin Bodi Chemical Holding Co., Ltd. Dulbecco׳s modified eagle׳s cell culture media (DMEM), Roswell Park Memorial Institute (RPMI) 1640 media, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and fetal calf serum (FCS) were purchased from Gibco-BRL (Grand Island, USA).

Wang F., Liu X., Liu C., Liu Z, & Sun L. (2015). Effects of antibiotic antitumor drugs on nucleotide levels in cultured tumor cells: an exploratory method to distinguish the mechanisms of antitumor drug action based on targeted metabolomics. Acta Pharmaceutica Sinica. B, 5(3), 223-230.

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