Ntera 2

PC-3, HCC15, NTERA-2, MCF-7, and LNCaP cells were purchased from DSMZ-German Collection of Microorganisms and Cell Cultures GmbH. The human TC cell line 1889c was kindly provided by Ehemann et al. [16 (link)]. Cells lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine, and 100 U/mL penicillin/streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a 5% CO₂ humidified environment.

EwS cell lines were grown as adherent cultures on collagen-coated flasks in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (Thermo Fisher, Dreieich, Germany) and 2 mM L-glutamin (Sigma-Aldrich, Taufkirchen, Germany) and maintained at 37 °C and 5% CO₂. TC-32, TTC-466, 5838, A4573 were gifts from the Children’s Hospital Los Angeles, United States. CADO-ES-1 (#ACC 255), RD-ES (#ACC 260), TC-71 (#ACC 516) and NTERA-2 (#ACC 527) were purchased from DSMZ (Braunschweig, Germany). A673 (#CRL-1598) and SK-N-MC (#HTB-10) from ATCC (Manassas, Virginia, USA). Cell lines MS-EwS-6, MS-EwS-15 and MS-EwS-16 were established in our institution, as reported previously^{31 (link)}, and WE-68 and VH-64 were gifts from the Institute of Experimental Orthopedics of our institution. Control cell lines SUP-B15 (B lineage leukemia) (#ACC 389) and HT-1080 (fibrosarcoma) (#ACC 315) were purchased from DSMZ and were cultivated in RMPI 1640 medium supplemented with 10% heat-inactivated FBS and 2 mM l-glutamin. Short tandem repeat (STR) profiling was used to confirm the identity of all cell lines (Supplementary Table 1), and all cell lines were regularly checked by PCR to exclude mycobacterial contamination.