Donepezil was provided by Eisai China Inc. (Suzhou, China). Aβ25-35, dimethyl sulfoxide (DMSO), a mouse monoclonal anti-β-actin antibody and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were provided by Sigma-Aldrich (St. Louis, MO, USA). The protein kinase C (PKC) inhibitor GF109203X was provided by Calbiochem (San Diego, CA, USA). A rabbit polyclonal anti-phospho-PKC (P-PKC) antibody and a rabbit polyclonal anti-phospho-myristoylated alanine-rich C kinase (MARCKS) antibody were provided by Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal anti-PKCα and PKCε antibodies were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). A Dulbecco’s modified Eagle medium (DMEM), foetal bovine serum (FBS), penicillin, streptomycin and pancreatin-EDTA (ethylene diamine tetraacetic acid) mixture was provided by Gibco (Carlsbad, CA, USA). RIPA cell lysis buffer was provided by Shenneng Bocai (Shanghai, China). A BCA protein assay kit was provided by Pierce (Pierce Biotechnology, Rockford, IL, USA). An ECL Western Blotting Detection kit was provided by Amersham-Pharmacia Biotech (GE Healthcare, Little Chalfont, UK).
Pancreatin edta
Manufactured by Thermo Fisher Scientific
Sourced in United States
Pancreatin-EDTA is a laboratory reagent used for cell detachment and dissociation. It contains a mixture of the proteolytic enzyme pancreatin and the chelating agent EDTA (Ethylenediaminetetraacetic acid). This combination helps to break down the extracellular matrix and cell-cell adhesions, enabling the release and separation of cells from a substrate or culture surface.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Mouse monoclonal anti β actin antibody, by Merck Group (1 mentions)
Ecl western blotting detection kit, by Cytiva (1 mentions)
Donepezil, by Eisai (1 mentions)
Beas 2b, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Solarbio (1 mentions)
2 protocols using pancreatin edta
1
Donepezil Neuroprotection Against Amyloid-Beta
2
Culturing Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Normal bronchial cell line BEAS-2B (CBP60577) and human LUAD cell lines A549 (CBP60084), H1299 (CBP60053), H522 (CBP60140), PC9 (CBP60078) and H1703 (CBP60115) were purchased from Nanjing Cobioer Biotechnology Co., LTD. BEAS-2B cells were cultured in serum-free bronchial epithelial cell growth medium (BEGM), and LUAD cells were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS; Hyclon, USA). The A549 cell line was frozen in a liquid nitrogen tank with 90% complete medium+10% dimethylsulfoxide (DMSO; Solarbio, USA), and the other experimental cell lines were frozen in the liquid nitrogen tank with 90% FBS+10% DMSO freezing medium. The resuscitated cells were cultured in a humid incubator at 37°C with 5% CO2. Cells were digested with 0.25% pancreatin-EDTA (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and passaged at 1:3 at 80%–90% in confluence. Logarithmic growth stage cells were collected for subsequent experiments.
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