The apparatus was similar to that used by Reyes-Mendez et al. [44 (link)], which consisted of a dark (black) acrylic enclosure, with a 30 × 30 cm floor area and a 40 cm height surrounding wall. We investigated the mouse in an open field cage for 7 min, which consisted of squares. The video data were analyzed for mouse behavior using a Daniovision video tracking system (Noldus, Wageningen, The Netherlands). The speed and total movement distances (cm) were calculated and analyzed using the Etho Vision XT software (Noldus). We performed three independent experiments and averaged three values.
Daniovision video tracking system
Manufactured by Noldus
Sourced in Netherlands
The DanioVision video-tracking system is a laboratory equipment product designed for the purpose of recording and analyzing the behavior of small aquatic organisms, such as zebrafish (Danio rerio). The system utilizes high-resolution video cameras to capture the movements and activities of the organisms within a controlled environment.
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Lab products found in correlation
4 protocols using daniovision video tracking system
1
Open Field Exploration in Mice
2
Behavioral Effects of CPPF on Zebrafish Larvae
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Individual 5 DPF eggs were transferred into wells of a 96 square well plate containing 300 µl of embryo medium and left to adapt for 1 h at 28 °C. Then 0 to 10-µM CPPF was added to a final medium volume of 400 µl (n = 6). Larva behaviors were tracked using the DanioVision video tracking system (Noldus, Wageningen, Netherlands) and total distances moved (mm) were measured and analyzed using the Etho vision XT software (Noldus).
In the color preference test, larvae were placed in a two-color maze chamber (yellow/blue). Ten of each larva per group were treated in 0 to 10 µM CPPF in 3 mL of embryo medium after incubation for 1 h in the dark. After adaptation, the larvae were exposed to 1 h of 123 lux light and recorded. Every 10 min, fish in the blue area were counted and analyzed.
In the color preference test, larvae were placed in a two-color maze chamber (yellow/blue). Ten of each larva per group were treated in 0 to 10 µM CPPF in 3 mL of embryo medium after incubation for 1 h in the dark. After adaptation, the larvae were exposed to 1 h of 123 lux light and recorded. Every 10 min, fish in the blue area were counted and analyzed.
3
Behavioral Tracking of pcdh19 Mutant Larvae
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At 6dpf larvae with pcdh19 mutations were transferred into wells (one each) of a 96-well plate, placed into the DanioVision video-tracking system (Noldus, Wageningen, Netherlands), and tracked with the Ethovision software after a 20-min acclimation period. For 60 min, baseline swim patterns were recorded and evaluated for seizure-like episodes.
4
Behavioral Tracking of pcdh19 Mutant Larvae
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