Anti tetra his

For western blotting analysis, proteins were separated by SDS-PAGE, and bands were visualized using the Odyssey infrared imaging system (LI-COR). Antibodies used for western blotting were anti-tetra-HIS (1:1000, Qiagen, #34670), anti-GST-Z5 (1:1000, Santa Cruz, #sc-459), anti-Tubulin (1:10,000, Sigma, #T6199), anti-H3 (1:5000 Abcam, #ab1791), anti-ARH3 (1:1000, custom made, Genosphere Biotech), IRDye 800CW goat anti-rabbit IgG (1:15,000, LI-COR, P/N 925-32211), and IRDye 680RD Goat anti-Mouse IgG (1:15,000, LI-COR, P/N 925-68070). Molecular weights are indicated by the PageRuler Plus Prestained Protein Ladder (Thermo Scientific). Uncropped western blottings are displayed in Supplementary Fig. 4.

Immunoblotting was performed using the following primary antibodies: anti-GFP (JL8 antibody catalog no. 632380, Takara; 1:1000), anti-PGK (catalog no. 459250, Invitrogen; 1:20,000), anti-FLAG (F3165, Sigma; 1:10,000), anti-Sts1 (^{11 (link)}; 1:5000), anti-Tetra-His (catalog no. 34670, Qiagen; 1:4000), anti-Rpn3 (catalog no. ab79769, Abcam; 1:5000), and anti-GST (catalog no. ab19256, Abcam; 1:10,000). Either donkey anti-rabbit IgG linked to horseradish peroxidase or sheep anti-mouse IgG linked to horseradish peroxidase (catalog no. NA934V and catalog no. NXA931V GE Healthcare, respectively) was used as the secondary antibody. Proteins were visualized on film (catalog no. E3018, Thermo Fisher Scientific) or with a G-box (SynGene) for quantification using enhanced chemiluminescence (ECL).