Dox hcl

The mPEG₄₀₀₀ was purchased from Best-reagent (Beijing, China). N-Carbobenzoxy-l-glutamic acid (PBLG) was purchased from Wuxi Asiapeptide Biotechnology Co., Ltd. (Wuxi, China). Acryloyl chloride, cysteamine hydrochloride, and DOX·HCl were purchased from Macklin (Shanghai, China). 4,6-Diamidino-2-phenylindole (DAPI), 1,10-dioctadecyl-3,3,30,30 tetramethylindodicarbocyanine perchlorate (DiD), and LysoTracker Green were purchased from Invitrogen (Carlsbad, CA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Hoechst 33342 was purchased from BD Biosciences (San Jose, CA).

Low molecular weight chitosan (LC) with a 5 kDa molecular weight was bought from Golden-Shell Pharmaceutical Co., Ltd. (Taizhou, China) and used as received. N, N’-carbonyldiimidazole (CDI), Cys·2HCl (97%), OA (99%), DA (98%), CA (99%), Dox·HCl (98%), triethylamine (TEA, 98%) and pyrene (97%) were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Hoechst 33342, paraformaldehyde (PA, 4%), MTT and GSH were procured from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were provided by Gibco BRL (Grand Island, NY, USA). Normal mouse fibroblasts cells (L929) and human lung cancer cells (A549) were provided by the Cell Bank of the Chinese Academy of Science (Shanghai, China).