Anti gapdh polyclonal

Manufactured by Abcam

Anti-GAPDH polyclonal is a laboratory reagent used for the detection and quantification of GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) in biological samples. GAPDH is a widely used housekeeping gene and protein for various applications in molecular biology and biochemistry.

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Lab products found in correlation

Anti src rabbit monoclonal, by Cell Signaling Technology (2 mentions) Anti phospho src tyr 416 rabbit polyclonal, by Cell Signaling Technology (2 mentions) Glutathione sepharose 4b beads, by GE Healthcare (2 mentions) Protein a g plus agarose bead, by Santa Cruz Biotechnology (2 mentions) Puromycin, by Merck Group (2 mentions) Ni2 nta agarose bead, by Qiagen (1 mentions) Saracatinib, by Selleck Chemicals (1 mentions) Anti ago2, by Cell Signaling Technology (1 mentions) Anti dicer, by Cell Signaling Technology (1 mentions) Anti myc, by Merck Group (1 mentions) Edta free protease inhibitor cocktail, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-GAPDH polyclonal antibody Rabbit polyclonal anti‐GAPDH Rabbit polyclonal anti-GAPDH antibody Anti-GAPDH GAPDH

2 protocols using anti gapdh polyclonal

Src and FAK Signaling Pathway Analysis

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Anti-Src rabbit monoclonal(#2109),anti-phospho-Src (Tyr 416) rabbit polyclonal (#2101; used for detecting human Src pY419), anti-phospho-Src (Tyr 527) rabbit polyclonal (#2105; used for detecting human Src pY530), anti-FAK rabbit polyclonal(#3285), anti- phospho-FAK (Tyr 925)rabbit polyclonal(#3284), Anti-Csk rabbit monoclonal(#4980) antibody were purchased from Cell Signaling Technology. Anti-GAPDH polyclonal (#ab37168), SUMO1 (#ab32058), Senp1 (#ab108981) antibody were from Abcam. Protein A/G PLUS Agarose beads (#K0115) were obtained from Santa Cruz Biotechnology. Ni²⁺-NTA agarose beads were from Qiagen. Glutathione Sepharose 4B beads (#17–0756-01) were from GE Healthcare Life Sciences. Puromycin (#P8833) was from Sigma. Anti-RPTPα (#7–091) is kept in our lab [28] (link).

Wang J., Deng R., Cui N., Zhang H., Liu T., Dou J., Zhao X., Chen R., Wang Y., Yu J, & Huang J. (2017). Src SUMOylation Inhibits Tumor Growth Via Decreasing FAK Y925 Phosphorylation. Neoplasia (New York, N.Y.), 19(12), 961-971.

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Antibody Detection Protocols for Src, AGO2, and Dicer

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Anti-Src rabbit monoclonal (#2109), anti-phospho-Src (Tyr 416) rabbit polyclonal (#2101; used for detecting human pY419 Src), anti-AGO2 (#2897) and anti-Dicer (#3363) antibodies were obtained from Cell Signaling Technology. anti-AGO2 (ab57113), anti-phospho-AGO2 (pY393 AGO2) (ab215746), anti-Tubulin (#66031-1-Ig), and anti-GAPDH polyclonal (#ab37168) antibodies were acquired from Abcam. Anti-Flag (M2), anti-HA and anti-Myc antibodies were purchased from Sigma-Aldrich. Protein A/G PLUS Agarose beads (#K0115) were obtained from Santa Cruz Biotechnology. Glutathione Sepharose 4B beads (#17-0756-01) were from GE Healthcare Life Sciences. Puromycin (#P8833) and EDTA-free Protease Inhibitor Cocktail were acquired from Sigma. Saracatinib (AZD0530) was purchased from Selleck.

Liu T., Zhang H., Fang J., Yang Z., Chen R., Wang Y., Zhao X., Ge S., Yu J, & Huang J. (2020). AGO2 phosphorylation by c-Src kinase promotes tumorigenesis. Neoplasia (New York, N.Y.), 22(3), 129-141.

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