Scleroglucan

Recombinant mouse GM-CSF, TNF-α, IL-1β, and IL-4 were purchased from Peprotech. Recombinant mouse IL-33 and human TGF-β were purchased from R&D Systems. OVA (323–339, ISQ AVH AAH AEI NEA GR) peptide used in OT-II mouse model and TRP-1 (106–130, SGH NCG TCR PGW RGA ACN QKI LTV R) peptide were purchased from GL Biochem (Shanghai) Ltd. Functional anti-mouse CD3e and CD28 antibodies (mAbs) were purchased from eBioscience. ST2-blocking mAb (αST2), IL-33 neutralization mAb (αIL33), IL-9 neutralization mAb (αIL9), and control IgG were purchased from R&D Systems. Curdlan and Scleroglucan were purchased from Sigma-Aldrich and Invivogen, respectively.

Recombinant mouse GM-CSF, TNF-α, IL-1β, IL-4, IL-9 and human TGF-β were purchased from Peprotech. TNFSF15 was purchased from R&D Systems. MHC class II restricted OT-II OVA peptide (OVA323–339, ISQAVHAAHAEINEAGR) was purchased from Genscript. Functional anti-mouse CD3e and CD28 antibodies (mAbs) were purchased from eBioscience. Dectin-1-blocking mAb was purchased from Invivogen. TNFSF15 neutralization mAb and OX40L-blocking mAb were purchased from R&D Systems. Curdlan and Scleroglucan were purchased from Sigma-Aldrich and Invivogen, respectively. Piceatannol (a Syk inhibitor) and GW5074 (a Raf1 kinase inhibitor) were purchased from Calbiochem. Bortezomib, a NF-κB inhibitor, was purchased from Selleckchem.