Hippocampus tissues were lysed using tissue lysis buffer for protein extraction. Protein concentration was quantified via the BCA method (Beyotime, China), then separated by 12% SDS-PAGE gels (Meilunbio, China) and transferred to PVDF membranes (0.22 μm, Millipore, America). The membranes were blocked with 5% non-fat milk powder for 1 h, then incubated with primary antibodies α7nAChR (1:1000, Affinity Biosciences, China), Nrf2 (1:1000, CST, China), HO-1 (1:1000, CST, China), CREB (1:1000, Affinity Biosciences, China), BDNF (1:1000, abcam, China), PSD95 (1:1000, CST, China) at 4 °C overnight. The membranes were washed thrice with TBST for 5 min, then incubated with goat anti-rabbit IgG (H+L) secondary antibody (1:10000, Thermo Fisher, America) at 4 °C for 1 h. Finally, the membranes were scanned using Odyssey system (LI-COR, America).
α7nachr
Manufactured by Affinity Biosciences
Sourced in China
The α7nAChR is a laboratory equipment product that functions as a nicotinic acetylcholine receptor. It plays a role in various biological processes, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Brain derived neurotrophic factor (bdnf), by Abcam (1 mentions)
Goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Odyssey system, by LI COR (1 mentions)
Bca method, by Beyotime (1 mentions)
Lymphocyte separation solution, by Solarbio (1 mentions)
Anti rat cd4 pe, by BioLegend (1 mentions)
Acetylcholinesterase ache assay kit, by Nanjing Jiancheng (1 mentions)
Biotin conjugated secondary antibodies, by Beyotime (1 mentions)
Cd86 fitc, by BioLegend (1 mentions)
Pe cd11b antibody, by BioLegend (1 mentions)
Ifn γ fitc, by BioLegend (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using α7nachr
1
Hippocampal Protein Expression Analysis
2
Microglia Regulation by MG in LPS-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MG (purity >98%) was purchased from SanHerb Bioscience (Chengdu, Sichuan, China). Carboxyl methyl cellulose sodium (CMC-Na) was obtained from Aladdin (Shanghai, China). Lipopolysaccharide (LPS, from the Gram-negative bacterium E. coli 055:B5) and methyllycaconitine (MLA, α7nAChR specific inhibitor) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Roswell Park Memorial Institute (RPMI) 1640 medium and Dulbecco’s modified Eagle’s medium (DMEM) were bought from Hyclone (Logan City, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco (CA, USA). Lymphocyte separation solution was bought from Solarbio (Beijing, China). Anti-rat CD4-PE, IFN-γ-FITC, CD86-FITC and CD11b-PE antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-rat p65, ac-p65, p-p65, α7nAChR and SIRT1 antibodies were the products of Affinity Biosciences (Changzhou, Jiangsu, China). Biotin-conjugated secondary antibodies were supplied by Beyotime Biotech (Nantong, Jiangsu, China). Acetylcholinesterase (AChE) assay kit and Coenzyme I NAD (H) assay kit were supplied by JianCheng Bioengineering Institute (Nanjing, Jiangsu, China).
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