Glutathione (gsh)

Manufactured by Maclin Biochemical Technology

Sourced in China

The GSH is a piece of lab equipment designed for the measurement of glutathione (GSH) levels in biological samples. It provides a reliable and accurate method for quantifying this important antioxidant molecule, which plays a crucial role in cellular processes. The GSH operates using standardized protocols and provides consistent results, making it a valuable tool for researchers and scientists working in the field of biochemistry and related disciplines.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using glutathione (gsh)

Colorimetric Assay for Amino Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents from commercial sources were used as received without further purification or modification unless otherwise stated. Dopamine hydrochloride (DA), o‐phenylenediamine (oPD), and FeCl₃·6H₂O were obtained from Sigma‐Aldrich. The amino acids glycine (Gly), valine (Val), lysine (Lys), serine (Ser), leucine (Leu), phenylalanine (Phe), glutamine (Gln), isoleucine (Ile), tyrosine (Tyr), tryptophan (Trp), threonine (Thr), methionine (Met), glutamate (Glu), alanine (Ala), aspartic acid (Asp), proline (Pro), glutamic acid (Glu), arginine (Arg), histidine (His), cysteine (Cys), asparagine (Asn), homocysteine (Hcys), vitamin C (Vc), and glutathione (GSH) were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Sodium chloride (NaCl), sodium hydrogen phosphate (Na₂HPO₄), and sodium phosphate monobasic (NaH₂PO₄) were obtained from Shanghai Lingfeng Chemical Reagent Co. Ltd. Omeprazole enteric capsules were purchased from Youcare Pharmaceutical Group Co. Ltd. (Beijing, China). Cell Counting Kit‐8 was purchased from Mycos Biotechnology Co., LTD (Shenzhen, China). Fluorescent dye IR‐26 was purchased from Qiyue Biological Technology Co. Ltd (Xian, China).

Ci Q., Wang Y., Wu B., Coy E., Li J.J., Jiang D., Zhang P, & Wang G. (2023). Fe‐Doped Carbon Dots as NIR‐II Fluorescence Probe for In Vivo Gastric Imaging and pH Detection. Advanced Science, 10(7), 2206271.

+ Open protocol

+ Expand

Synthesis and Characterization of Metal-Polymer Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carboxymethylcellulose (CMC, M. W. 90 000, 250 000, 700 000 g mol⁻¹), 1,2-propanediamine and 1,6-hexamethylenediamine were procured from Aladdin Chemistry Co., Ltd. Ethylenediamine (EDA, 99% purity) was purchased from Kermel Analytical Reagent Co., Ltd. CrCl₃·6H₂O, MgCl₂, AgNO₃, KCl, MnCl₂·4H₂O, NaCl, ZnCl₂, BaCl₂·2H₂O, AlCl₃, FeCl₃·6H₂O, CuCl₂·2H₂O, CaCl₂, FeCl₂·4H₂O were obtained from Sinopharm Chemical Reagent Co., Ltd. K₂CrO₄, K₂Cr₂O₇, KMnO₄, TiCl₃, glutathione (GSH), ascorbic acid, SnCl₄ and hydroxylamine hydrochloride were supplied by Macklin Biochemical Co., Ltd.

Liu Z., Li R., Li Y., Zhou J., Gong Y., Shi H., Guo Y., Li H., Wang Z, & Zhang F. (2021). Fluorescent N-functionalized carbon nanodots from carboxymethylcellulose for sensing of high-valence metal ions and cell imaging. RSC Advances, 11(55), 34898-34907.

+ Open protocol

+ Expand

Quantifying SCP2 Binding to 15(S)-HpETE

Check if the same lab product or an alternative is used in the 5 most similar protocols

The binding rate of SCP2 and 15(S)–HpETE (a monohydroperoxy polyunsaturated fatty acid in cells) was determined by a Fast Micro-Equilibrium Dialyzer (Harvard Apparatus) and LC-MS/MS (AB sciex, API 4000 Q-Trap). Recombinant SCP2 (5 μM; Sangon, Shanghai, China) and 15(S)–HpETE (5 μM; Glpbio, CA, USA) were mixed with or without ScpI2 (5 μM) in phosphate buffered saline (PBS; Corning, NY, USA) and then filled in one chamber. The other chamber was filled with blank PBS. A 3.5 kDa dialysis membrane (Viskase, USA) was applied to separate the chambers which were dialyzed for 2 h at 37 °C in a shaker. A mixture of SCP2 (5 μM) and GSH (5 μM; Macklin, Shanghai, China) served as negative control. After dialysis, the solution from both sides was subjected to compound extraction and LC-MS/MS analysis.

Dai T., Xue X., Huang J., Yang Z., Xu P., Wang M., Xu W., Feng Z., Zhu W., Xu Y., Chen J., Li S, & Meng Q. (2023). SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis. Cell Death Discovery, 9, 234.

+ Open protocol

+ Expand

Doxorubicin-Loaded Silica Nanoparticles for Cancer Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Doxorubicin (DOX), glutathione (GSH), 3-aminopropyltriethoxysilane
(APTES), tetraethyl orthosilicate (TEOS), bis[3-(triethoxysilyl)propyl]disulfide, N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride (TSA), 2,3-dimethylmaleic
anhydride (DMA), cetyltrimethylammonium bromide (CTAB), fluorescein
isothiocyanate (FITC), hyaluronic acid (HA, M_w = 10 kDa), and ammonium hydroxide were obtained from Sigma-Aldrich.
All of the chemicals and reagents used in this study were of analytical
grade and used as received without further purification. GSH was obtained
from Shanghai Macklin Biochemical Co., Ltd., and HAase was purchased
from Sigma-Aldrich.

Jin R., Liu Z., Bai Y., Zhou Y, & Chen X. (2018). Multiple-Responsive Mesoporous Silica Nanoparticles for Highly Accurate Drugs Delivery to Tumor Cells. ACS Omega, 3(4), 4306-4315.

+ Open protocol

+ Expand

Synthesis and Characterization of Metal Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

GSH was purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). DL-Dithiothreitol (DTT, 99%) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Phosphate buffered saline (PBS) was purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). Sodium citrate (Na₃C₆H₅O₇.2H₂O), chloroauric acid tetrahydrate (HAuCl₄-4H₂O), iron chloride (FeCl₃), lead chloride (PbCl₂), aluminum chloride (AlCl₃), magnesium chloride (MgCl₂), barium chloride (BaCl₂), cobalt chloride (CoCl₂), calcium chloride (CaCl₂), sodium chloride (NaCl), and mercury chloride (HgCl₂) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). SMF (core/cladding diameter, 8/125 μm) and MMF (core/cladding diameter, 50/125 μm) were purchased from Yangtze Optical Fiber and Cable Co., Ltd. (Wuhan, China). Deionized (DI) water was used to prepare the solution for this study.

Wang J., Niu K., Hou J., Zhuang Z., Zhu J., Jing X., Wang N., Xia B, & Lei L. (2023). Advanced Integration of Glutathione-Functionalized Optical Fiber SPR Sensor for Ultra-Sensitive Detection of Lead Ions. Materials, 17(1), 98.

+ Open protocol

+ Expand

Amino Acid Isolation and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

BSA (fraction V, heat shock isolation) was purchased from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). AA was obtained from Tianjin Kermel Chem reagent Co., Ltd. (Tianjin, China). Phosphate buffer saline (PBS) was prepared in this experiment. Phe was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Pro was purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glu, Ser, Asp, Val, Leu, Thr, glucose, sucrose, fructose and GSH were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All amino acids are L-type. All other chemicals were of analytical grade and used without further purification. The ultrapure water (18.2 MΩ cm) was obtained by the Milli-Q purification system (Milipore, Germany).

Song J., Guo X., Chen H., Tang Y, & Han L. (2023). Ascorbic Acid-Caused Quenching Effect of Protein Clusteroluminescence Probe: The Fast Fluorescent Detection of Ascorbic Acid in Vegetables. Molecules, 28(5), 2162.

+ Open protocol

+ Expand

Sensitive E. coli O157:H7 Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Biotin-3-sulfo-N-hydroxysuccinimide ester sodium salt, streptavidin-ALP (SA-ALP), ALP, HAuCl 4 •3H 2 O, AgNO 3 , Tween-20, amylose, AAP, ascorbic acid, and BSA were bought from Sigma-Aldrich Chemical Co., and KIO 3 and NaI were purchased from Aladdin. The GSH and I 2 were bought from Macklin. Anti-Escherichia coli O157:H7 polyclonal antibody (pAb) and anti-E. coli O157:H7 mAb were purchased from Zodolabs Bioengineering Co. Ltd. All other chemical reagents were of analytical grade and obtained from Sinopharm Chemical Reagent Co. Ltd. Ultrapure water (18.2 MΩ, 25°C) was obtained from a Milli-QA apparatus. The milk samples produced by the Inner Mongolia Yili Industrial Group Co. Ltd. were bought from a local Rainbow supermarket. The ELISA kits for E. coli O157:H7 detection were purchased from Zodolabs Bioengineering Co. Ltd.

Fang B., Peng J., Zhang G., Xing K., Chen W., Liu D., Shan S., Xiong Y, & Lai W. (2022). I₂/I^--mediated fluorescence quenching of an Ag⁺-doped gold nanocluster-based immunoassay for sensitive detection of Escherichia coli O157:H7 in milk. Journal of dairy science, 105(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!