Blood samples were collected every three days (d1, 4, 7, 10, 13, 16, 19, 22, 25, and 28) at 1400 h from jugular venepuncture using non-heparinized vacutainers (20 mL; Becton–Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton–Dickinson, Franklin Lakes, NJ, USA)21 (link). Serum samples were obtained from blood after centrifugation at 2700× g at 4 °C for 15 min. Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at − 80 °C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PIII, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PIIIs, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
Dri chem 7000i biochemistry analyzer
Manufactured by Fujifilm
Sourced in Japan
The DRI CHEM 7000i is a biochemistry analyzer manufactured by Fujifilm. It is designed for the quantitative analysis of various biochemical components in biological samples. The instrument utilizes dry chemistry technology to perform a range of tests, including the measurement of enzymes, proteins, lipids, and other analytes. The DRI CHEM 7000i is capable of delivering accurate and reliable results, but a detailed description of its intended use or performance characteristics is not available.
Automatically generated - may contain errors
Lab products found in correlation
1.5 ml tube, by Eppendorf (2 mentions)
Free fatty acids kits, by Roche (2 mentions)
7600 chemistry autoanalyzer, by Hitachi (2 mentions)
Bovine elisa test kit, by Life Diagnostics (2 mentions)
Fuji dri chem slide glu p 3, by Fujifilm (1 mentions)
Multiplex biomarker immunoassays for luminex xmap technology, by Merck Group (1 mentions)
Tbars, by Cayman Chemical (1 mentions)
4 protocols using dri chem 7000i biochemistry analyzer
1
Serum Biomarker Analysis in Blood Samples
2
Biomarker Analysis of Metabolic Hormones
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Serum levels of glucose and ketone bodies were measured using a Drichem 7000i biochemistry analyzer (Fujifilm, Tokyo, Japan) and an AutoWako Total Ketone Bodies clinical assay kit (Wako, Osaka, Japan), respectively. Serum levels of adiponectin, insulin, C‐peptide 2, leptin, resistin, and gastric inhibitory polypeptide (GIP; also known as glucose‐dependent insulinotropic polypeptide 19 (link)) were measured using Multiplex Biomarker Immunoassays for Luminex xMAP technology (Millipore, Billerica, MA, USA); Catalog Nos. MADPNMAG‐70K‐01 for adiponectin; and MMHMAG‐44k for other hormones. Quantitative analyses were performed using Luminex xPONENT and MILLIPLEX Analyst 4.2 software.
3
Serum Biomarkers for Oxidative Stress
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Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were measured using a Dri-Chem 7000i biochemistry analyzer (Fujifilm, Tokyo, Japan). Serum levels of malondialdehyde (MDA) were measured using a TBARS (thiobarbituric acid-reactive substances) assay kit (Cayman Chemical, Ann Arbor, MI, USA) (Yamada et al., 2012) .
4
Comprehensive Blood Sampling and Analysis
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Blood samples were collected every three days (d1, 4, 7, 10, 13, 16, 19, 22, 25, and 28) at 1400 h from jugular venepuncture using non-heparinized vacutainers (20 mL; Becton-Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton-Dickinson, Franklin Lakes, NJ, USA) 20 . Serum samples were obtained from blood after centrifugation at 2,700 × g at 4°C for 15 minutes.
Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
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