Protein lysates were sonicated and subjected to Tris-glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to nitrocellulose membranes. Membranes were then blocked for 1 h with 5% nonfat milk, incubated with primary antibody overnight and secondary antibody for 1 h. The primary antibodies included: anti-Id3 (C-20; Santa Cruz Biotechnology), anti-GAPDH (EMD; Millipore, Billerica, MA), anti-active caspase-3 (Enzo Life Sciences, Inc., Farmingdale, NY), anti-caspase-8 (Thermo Scientific Rockford, IL), and anti-Elk-1 (I-20; Santa Cruz Biotechnology).
Anti elk 1 1 20
Manufactured by Santa Cruz Biotechnology
Anti-Elk-1 (I-20) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that specifically recognizes the Elk-1 protein. The Elk-1 protein is a transcription factor that plays a role in regulating gene expression.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti elk 1 1 20
1
Immunoblot Analysis of Apoptosis Markers
2
ChIP-qPCR Assay for RUNX1 and Elk-1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP assay was performed as previously described 18 . Briefly, chromatin from crosslinked cells (20 x 10 6 HL-60 cells and 70 x 10 6 in Jurkat cells per condition) was sonicated, incubated overnight with goat anti-RUNX1 (C-19), rabbit anti-Elk-1 (I-20) (Santa Cruz Biotechnology, Inc.) and goat (RUNX1 IP) and rabbit (Elk-1 IP) anti-IgG antibodies (Sigma-Aldrich) in RIPA buffer, and precipitated with protein G/A-Sepharose. Cross-linkage of the co-precipitated DNA-protein complexes was reversed, and DNA was used as a template for quantitative PCR (qPCR). Primers employed are shown in Supplementary Table 3.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!