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Her2 pe

Manufactured by BioLegend
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HER2-PE is a fluorescently labeled antibody that specifically binds to the HER2 receptor. It can be used for flow cytometry applications to detect and quantify HER2 expression on cells.

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Lab products found in correlation

Cytoflex s flow cytometer, by Beckman Coulter (1 mentions) Live dead fixable aqua, by Thermo Fisher Scientific (1 mentions) Muc1 pe cy7, by BioLegend (1 mentions) Cd49f apc, by BioLegend (1 mentions) Quantibrite beads, by BD (1 mentions)

Spelling variants of this product

PE anti-human CD340 (ErbB2/HER2) antibody (3 citations) PE conjugated antihuman CD340 (erbB2/HER2) antibody (2 citations) PE)-labeled anti-human HER2 (24D2) (2 citations)

3 protocols using her2 pe

1

Co-culture Bystander Cytotoxicity Assay

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For the co-culture–based bystander experiment, 10.000 HER2-positive SKBR-3 and 2.000 HER2-negative MDA-MB-468 cells were treated with trastuzumab-LP5 DAR8 or Enhertu at concentrations ranging from 0.05 to 3 μg/mL. After 5 days, cells were stained with LIVE/DEAD Fixable Aqua (Thermo Fisher Scientific) and HER2-PE (BioLegend) to distinguish between target-positive and -negative cells. The percentage of viable HER2-positive and -negative cells was analyzed by flow cytometry on a CytoFLEX S flow cytometer (Beckman Coulter).
Schmitt S., Machui P., Mai I., Herterich S., Wunder S., Cyprys P., Gerlach M., Ochtrop P., Hackenberger C.P., Schumacher D., Helma J., Vogl A.M, & Kasper M.A. (2023). Design and Evaluation of Phosphonamidate-Linked Exatecan Constructs for Highly Loaded, Stable, and Efficacious Antibody–Drug Conjugates. Molecular Cancer Therapeutics, 23(2), 199-211.
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2

Immunophenotyping of Breast Cancer Cells

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The autologous breast cancer cells were stained with HER-2 PE, epithelial cell adhesion molecule (Ep-CAM) PE-Dazzle 594, mucin-1 (MUC-1) PE-Cy7 and integrin alpha 6 (CD49f) APC (Biolegend, USA) as recommended by the manufacturer. The cells were acquired on the LSRII flow cytometer and the data were analysed as indicated previously.
Tomasicchio M., Semple L., Esmail A., Meldau R., Randall P., Pooran A., Davids M., Cairncross L., Anderson D., Downs J., Malherbe F., Novitzky N., Panieri E., Oelofse S., Londt R., Naiker T, & Dheda K. (2018). An autologous dendritic cell vaccine polarizes a Th-1 response which is tumoricidal to patient-derived breast cancer cells. Cancer Immunology, Immunotherapy, 68(1), 71-83.
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3

Quantification of Surface Receptor Expression

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Antibodies bound per cell (ABC) were determined by staining of cell lines with saturating concentrations of HER2-PE (BioLegend) or the respective isotype control and acquisition by flow cytometry on a CytoFLEX S flow cytometer. Quantification of ABCs as an estimation for surface receptor expression was performed by simultaneous acquisition of QuantiBRITE beads (BD Biosciences) conjugated with four levels of PE and generation of a standard curve.
Schmitt S., Machui P., Mai I., Herterich S., Wunder S., Cyprys P., Gerlach M., Ochtrop P., Hackenberger C.P., Schumacher D., Helma J., Vogl A.M, & Kasper M.A. (2023). Design and Evaluation of Phosphonamidate-Linked Exatecan Constructs for Highly Loaded, Stable, and Efficacious Antibody–Drug Conjugates. Molecular Cancer Therapeutics, 23(2), 199-211.
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