PBMC were transduced and expanded 9-days and stained to detect GzmB (BD Bioscience 560,213). PBMC were expanded an additional 9-days with media supplemented with solubilized anti-CD3 antibodies (OKT3) (50 ng/ml) and IL-2 (300 IU/ml). PBMC were re-stimulated with Cell Activation Cocktail with BFA for detection of IL-2 and IFN-γ (Biolegend).
Cell activation cocktail with bfa
Manufactured by BioLegend
The Cell Activation Cocktail with BFA is a solution designed to activate and stimulate cells in cell culture experiments. It contains a combination of reagents that promote cellular activation and protein secretion. The core function of this product is to facilitate the study of cellular responses and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cell activation cocktail with bfa
1
Expansion and Activation of PBMCs
2
Expanded PBMC Cytokine Production
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