The QIAGEN Human Angiogenesis PCRArray Kit was used to analyze angiogenesis‐related gene expression. Total RNA was extracted with TRIzol from A549/TARBP2 and A549/GFP cells, reverse‐transcribed to cDNA, and added to each well of the PCRArray plates combined with SYBRGreen qRT‐PCR MasterMix. Data analysis was based on the ΔΔCt method, with normalization to three different housekeeping genes.
Human angiogenesis pcrarray kit
Manufactured by Qiagen
The Human Angiogenesis PCRArray Kit is a laboratory tool designed to assess the expression of genes involved in the process of angiogenesis, the formation of new blood vessels, in human samples. The kit provides a comprehensive set of optimized primer assays to measure the mRNA levels of key angiogenesis-related genes in a standardized and efficient manner.
Automatically generated - may contain errors
3 protocols using human angiogenesis pcrarray kit
1
Angiogenesis-Related Gene Expression Profiling
2
Profiling Angiogenesis-Related Genes via qPCR
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Total RNA was extracted from tissues or cultured cells using TRIzol (Invitrogen) and reverse transcribed to cDNA for qPCR using SYBR Green Fast Master Mix (Roche). The expression level of each gene was normalized to the mRNA level of GAPDH based on the ∆∆Ct method. A QIAGEN Human Angiogenesis PCRArray Kit was used to analyze angiogenesis‐related gene expression in accordance with the manufacturer's instructions. All the PCR array data are listed in Table S2 .
3
Quantitative analysis of angiogenesis genes
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Total RNA was extracted with TRIzol from tissues or cultured cells and reverse transcribed to cDNA for qPCR using SYBR green Fast Master Mix (Roche). Gene expression level was based on the ∆∆Ct method and normalized to GAPDH. The QIAGEN Human Angiogenesis PCRArray Kit was used to analyze angiogenesis-related gene expression. Total RNA was extracted with TRIzol from MDA-MB-468/Roquin2 and MDA-MB-468/GFP cells, purified using the RNA PureLink Kit (Thermo Fisher Scientific), reverse transcribed to cDNA, and added to each well of the PCRArray plates combined with SYBRGreen qPCR MasterMix according to the manufacturer's instructions. Data analysis was based on the ΔΔCt method, with normalization to three different housekeeping genes. The primer sequences for qPCR and PCR were listed in Supplementary Table S3 .
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