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2 protocols using lipase b acrylic resin

1

Enzymatic Synthesis of Organic Compounds

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Octanoic acid (purity ≥ 99%), urea (98%), urea hydrogen peroxide (purity 97%), hydrogen peroxide 30 wt% solution in water were purchased from Sigma–Aldrich (USA). Choline chloride (purity 99%) was purchased from Acros organics (Belgium). Fructose (purity 99%) was purchased from Alfa Aesar (USA). Analytical grade chemicals: glucose, glycerol, sodium hydrogen carbonate, sodium thiosulphate, anhydrous magnesium sulphate, organic solvents were purchased from P.P.H. Stanlab (Poland), Chempur (Poland) and POCH (Poland) (Table S2). Lipase B acrylic resin from Candida antarctica (CAL-B AR, > 5000 U/g), Lipase B Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CAL-B I150 ≥ 1800 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), lipase from Rhizopus arrhizus (RAL, ~10 U/mg) and Amano Lipase PS from Burkholderia cepacia (Amano PS, ≥ 30,000 U/ g) were purchased from Sigma-Aldrich (USA).
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2

Enzymatic Activity Assays Development

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Trehalose dihydrate (#T0167), vinyl acrylate (#771422), Lipase B acrylic resin from Candida antarctica (CALB) (#L4777), trimethylopropane ethoxylate (#416177), ethyl thiolactate (#W327905), ethyl thioglycolate (#E34307), polyethylene diacrylate (PEGDA) (Mn = 575 g mol−1) (#437441), activated alumina Brockman I (basic and neutral), Filter agent Celite 545, solvents (dichloromethane, methanol, ethyl acetate, acetone, dimethyl sulfoxide), 3A and 4A molecular sieves, horseradish peroxidase (#77332, 156 U mg−1), glucose oxidase from Aspergillus Niger (#49180, 192 U mg−1), α-Chymotrypsin from bovine pancreas (#C3142), 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (#T0440), d-(+)-glucose anhydrous, N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (#S7388, chymotrypsin substrate), 2 n HCl solution, Tris hydrochloride (Tris-HCl), calcium chloride (CaCl2) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline pH = 7.4, FITC labeled ovalbumin, IgG-Alex Fluor 647 and Amplex UltraRed reagent were purchased from Life Technologies (Grand Island, NY, USA). Disposable 40 and 80 g HP Silica Gold Cartridges were purchased from Teledyne Isco (Lincoln, NE, USA).
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