Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), trypsin-ethylenediamine tetra acetic acid (EDTA), and streptomycin/penicillin were purchased from Welgene (Daegu, Korea) and Gibco (Grand Island, NY, USA). Anti-DNP-IgE, bovine serum albumin (BSA), p-nitrophenyl-N-acetyl-β-d -glucosaminide, DNCB, and 1-Iodo (1-iodohexadecane) were purchased from Sigma-Aldrich (St. Louis, MO, USA). DNP-BSA was purchased from Thermo Scientific (Waltham, MA, USA) and TNF-α from R&D Systems (Minneapolis, MN, USA). Anti-SNAP23, anti-syntaxin 4, anti-VAMP8, anti-LOR, and anti-FLG antibodies were from Abcam (Cambridge, MA, USA), and the anti-β-actin antibodies were from Sigma-Aldrich. Anti-rabbit IgG HRP-conjugated and anti-mouse IgG HRP-conjugated antibodies were from Cell Signaling (Danvers, MA, USA).
Anti flg
Manufactured by Abcam
Sourced in United States
Anti-FLG is a primary antibody that specifically recognizes the Filaggrin (FLG) protein. Filaggrin is a structural protein that plays a crucial role in the formation of the skin's protective barrier. This antibody can be used in various immunoassay techniques to detect and quantify Filaggrin expression in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti dsc1, by Novus Biologicals (2 mentions)
Anti krt10, by BioLegend (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Hrp conjugated anti mouse igg antibody, by Cell Signaling Technology (1 mentions)
Anti vamp8, by Abcam (1 mentions)
Anti dnp ige, by Merck Group (1 mentions)
Anti snap23, by Abcam (1 mentions)
P nitrophenyl n acetyl β d glucosaminide, by Merck Group (1 mentions)
Anti syntaxin 4, by Abcam (1 mentions)
Hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Dnp bsa, by Thermo Fisher Scientific (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Radioimmunoprecipitation assay buffer, by Merck Group (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase, by Santa Cruz Biotechnology (1 mentions)
Anti ki67, by Abcam (1 mentions)
3 protocols using anti flg
1
Mast Cell Degranulation Assay
2
Keratinocyte Protein Expression Analysis
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NHEKs were lysed in Radioimmunoprecipitation assay buffer (Millipore). After centrifugation at 13,000 rpm for 10 minutes at 4 C, the supernatant was loaded onto a 5e20% gradient gel and stained with the following antibodies: anti-KRT10 (Biolegend), anti-DSC1 (Novus), anti-FLG (Abcam), anti-glyceraldehyde-3-phosphate dehydrogenase (Santa Cruz Biotechnology, Santa Cruz, CA), antiphosphorylated and total forms of p44/42 MAPK, c-Jun N-terminal kinase, p38, and p65 of the NF-kB (Cell Signaling Technology, Beverly, MA).
3
Internalization of Labeled PEVs in NHEKs
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PEVs were labeled with DiI (Molecular Probes, Eugene, OR) as described in the Supplementary Material. NHEKs were treated with DiI-labeled PEVs with or without endocytic inhibitors, stained with an anti-early endosome antigen 1 antibody (Abcam, Cambridge, MA), and analyzed under a confocal microscope (LSM 700; Carl Zeiss, Jena, Germany). For immunohistochemical analysis, replicate sections from reconstituted human skin were stained with the following antibodies: anti-KRT10 (Biolegend, San Diego, CA), anti-DSC1 (Novus, Littleton, CO), anti-FLG (Abcam), and anti-Ki67 (Abcam). Detailed protocols for immunological analyses are described in the Supplementary Material.
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