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Proelut psa

Manufactured by Dikma Technologies
Sourced in United States, China

ProElut PSA is a lab equipment product designed for the purification of proteins from complex biological samples. It utilizes a column-based chromatography technique to selectively capture and elute target proteins.

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2 protocols using proelut psa

1

Polycyclic Aromatic Hydrocarbons Analysis

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The 16 PAHs’ standard mixture (1000 mg/L, purity ≥ 96%, hexane/acetone 1:1) and 5 PAHs’ isotope internal standard mixture (2000 mg/L, purity ≥ 98% methylene dichloride) were purchased from ANPEL Laboratory Technologies Inc. (Shanghai, China), including naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, dibenzo[a,h], anthracene, benzo[g,h,i]perylene, naphthalene-d8, acenaphthene-d10, phenanthrene-d10, chrysene-d12, and perylene-d12. PAH standard and isotope internal standard stock solutions were diluted in cyclohexane at a concentration of 10 µg/L. Three types of SPE columns, ProElut C18 (1 g/6 mL), ProElut Florisil (1 g/6 mL), and ProElut PSA (500 mg/6 mL) (Dikma Technologies, Radnor, PA, USA), were used for sample purification. All organic solvents, including acetonitrile, cyclohexane, and ethyl acetate were of HPLC grade (Dikma Technologies Inc., Radnor, PA, USA).
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2

Extraction and Analysis of Lutein

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All chemicals
were of analytical grade unless otherwise indicated. Potassium nitrate
(purity ≥99.0%) was purchased from Sinopharm (Beijing, China).
Lutein (HPLC ≥90%) and methanol (HPLC grade) were purchased
from Solarbio (Beijing, China) and Fisher Chemicals (Loughborough,
UK). Ultrapure water was produced by a Milli-Q purification system.
The SPE cartridge ProElut AC 250 mg/3 mL, ProElut C18 500 mg/3 mL,
ProElut CARB 250 mg/3 mL, ProElut CARB 500 mg/6 mL, ProElut NH2 500 mg/3 mL, ProElut PSA 500 mg/3 mL, ProElut CARB/NH2 500/500 mg/6 mL and ProElut CARB/PSA 500/500 mg/6 mL were
purchased from Dikma (Beijing, China).
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