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5 ml vacutainers

Manufactured by Greiner
Sourced in Germany

The 5 mL vacutainers are sterile, single-use laboratory specimen collection tubes designed to hold up to 5 milliliters of liquid samples. They are made of high-quality materials and feature a vacuum-sealed closure to ensure sample integrity during collection and transportation.

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3 protocols using 5 ml vacutainers

1

Plasma Separation from Whole Blood

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Approximately, 5 mL of the whole blood was taken by venipuncture into 5 mL vacutainers (Greiner Bio-One, Germany) containing EDTA. To avoid direct exposition to light, specimens were transported in cryoboxes. Plasma was separated after centrifugation of blood at 750g for 10 min at room temperature, and then stored at −80 °C until analysis.
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2

Blood Sample DNA Extraction

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Approximately, 5 ml of the whole blood was taken by venepuncture into 5 ml vacutainers (Greiner Bio‐One, Germany) containing EDTA. Genomic DNA was isolated from all peripheral blood samples by using a commercial kit (Isolate II Genomic DNA Kit, BIOLINE) according to manufacturer's recommendations. DNA obtained was immediately used for PCR amplification or stored at −20°C until use.
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3

Genomic DNA Isolation from Whole Blood

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Approximately 5 ml of whole blood was taken by venipuncture into 5-ml vacutainers (Greiner Bio-One, Germany) containing EDTA. Genomic DNA was isolated from all peripheral blood samples by using a commercial kit (Isolate II Genomic DNA Kit; BIO-LINE) according to the manufacturer's recommendations. The DNA obtained was immediately used for polymerase chain reaction (PCR) amplification or stored at -20 ° C until use.
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