Flex trs high

Manufactured by Agilent Technologies

The Flex TRS High is a versatile laboratory equipment product offered by Agilent Technologies. It serves as a core component for various analytical applications. The detailed technical specifications and intended use cases are not available as part of this unbiased and factual description.

Automatically generated - may contain errors

Lab products found in correlation

Omnis autostainer, by StatLab (4 mentions) Clearify, by StatLab (4 mentions) Rabbit linker, by Agilent Technologies (1 mentions) Caspase 3 antibody, by Cell Signaling Technology (1 mentions) Mouse linker, by Agilent Technologies (1 mentions)

4 protocols using flex trs high

Immunohistochemical Detection of VEGFR3

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 15 minutes. Slides were incubated with VEGFR3 antibody (BD Pharmigen; catalog# 552857) for 30 minutes at 1/40 (3.13 IgG). Rabbit Anti-Rat (Agilent; E0468) which was applied for 30 minutes followed by Rabbit Envision (Agilent K4003) for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then put into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.

Vroom J.M., De Grauw K.J., Gerritsen H.C., Bradshaw D.J., Marsh P.D., Watson G.K., Birmingham J.J, & Allison C. (1999). Depth Penetration and Detection of pH Gradients in Biofilms by Two-Photon Excitation Microscopy. Applied and Environmental Microbiology, 65(8), 3502-3511.

+ Open protocol

+ Expand

Caspase 3 Immunohistochemistry on FFPE Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Slides were incubated with Caspase 3 antibody (Cell Signaling, 9661) for 30 minutes at 1/50 (1.26 IgG). Rabbit Linker (Dako, GV809) was applied for 10 mins followed by Rabbit Envision (Dako #K4003) for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then placed into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.

+ Open protocol

+ Expand

Immunohistochemical Staining of CD163

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin and zinc fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Slides were incubated with CD163 antibody (Abcam, ab182422) for 30 minutes at 1/300 (2.24 IgG). Rabbit Envision (Dako #K4003) was applied for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then placed into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.

+ Open protocol

+ Expand

Immunohistochemical Staining of CD8+ T-Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

TMA sections were added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Sections were incubated with CD8 (Dako #M7103) for 10 minutes at 1/50. Mouse Linker (Dako #GV821) was applied for 10 minutes followed by horseradish peroxidase for 15 mins (Dako GV823). Diaminobenzidine (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with hematoxylin for 8 minutes then placed into water. Sections were dehydrated, cleared and coverslipped.

Yang Y., Attwood K., Bshara W., Mohler J.L., Guru K., Xu B., Kalinski P, & Chatta G. (2020). High intratumoral CD8+ T-cell infiltration is associated with improved survival in prostate cancer patients undergoing radical prostatectomy. The Prostate, 81(1), 20-28.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!