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Pseudomonas fluorescence

Manufactured by Merck Group
Sourced in Japan

Pseudomonas Fluorescence is a laboratory equipment designed to detect and quantify the presence of Pseudomonas fluorescens, a common environmental bacteria. The equipment utilizes the natural fluorescent properties of this bacteria to provide reliable and accurate identification and measurement.

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2 protocols using pseudomonas fluorescence

1

Sourcing Chemicals and Enzymes for Research

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We obtained various chemicals
for our research from different suppliers. Uridine, HMDS, imidazole,
hydroxylamine sulfate, and isobutyric anhydride were procured from
TCI Chemicals. Addzyme011, Addzyme 017, and Addzyme 022 were acquired
from Advance Enzyme Pvt. Ltd. Other enzymes like L-4447, Rhizopus niveus, Porcine pancreas, Mucor miehei, Rhizopus
oryzae
, Aspergillus niger, Pseudomonas Fluorescence, Pseudomonas Cepacia was sourced from SIGMA, while
CES L 1-8 was obtained from Amano Chem. Ltd. We purchased chemicals
and solvents necessary for our synthesis and extraction processes
from commercial sources such as SIGMA (located in St. Louis, Missouri,
USA), TCI Chemicals, and SRL Pvt. Ltd. For our HPLC analysis, we utilized
high-performance liquid chromatography (HPLC)-grade solvents that
were provided by Finar Limited.
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2

Waste Lollipop Oil to Biodiesel

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Feedstock used in this experiment was procured from a local lollipop unit located in North-West province, South Africa. KOH, CaO, Ca(OH)2, methanol, diethyl ether and deuterated chloroform (CDCl3) of analytical grades were purchased from Sigma-Aldrich and Associated Chemical Enterprises (ACE), Johannesburg, South Africa. Mucor miehei, Pseudomonas cepacia, Rhizopus delemar, Geotrichum candidum, Candida rugosa, Caprica papaya, Porcine pancreas-II, Pseudomonas fluorescence and Candida antarctica lipase-B (Novozyme-435) lipases were obtained from Sigma-Aldrich, Amano Enzyme Inc., Nagoya, Japan and from local enzyme suppliers of South Africa. Waste oil to biodiesel conversion (%) was calculated from 1 H NMR (Nuclear Magnetic Resonance) using a Bruker instrument (600 MHz) located at the Faculty of Natural Sciences, Laboratory for Analytical Services, North West University, Potchefstroom, South Africa.
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