Fetal bovine albumin

Manufactured by Thermo Fisher Scientific

Sourced in United States, Ireland

Fetal bovine albumin is a serum protein derived from the blood of fetal bovine origin. It is commonly used as a supplement in cell culture media to provide essential nutrients and growth factors for the cultivation of various cell types.

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Fetal bovine serum albumin (8 citations) Bovine albumin (7 citations) Fetal bovine serum albumin (FBS), (2 citations)

4 protocols using fetal bovine albumin

Neurotensin Receptor 1 Targeting Protocol

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Human IgG was purchased from Equitech Bio (Kerrville, TX, USA). Poloxamer-188, RNase-free water, 4,6-diamidino-2-phenylindole (DAPI), and fetal bovine albumin (FBS) were obtained from Fisher Scientific. Antineutotensin receptor 1-monoclonal antiboby (anti-NTSR1-mAb was purchased from Santa Cruz Biotechnologies. siRNA against mutated KRAS G12S was designed andpurchased from GE Dharmacon. siG12S sense and antisense sequences are GUUGGAGCUAGUGGCGUAGdTdT and CUACGCCACUAGCUCCAACdTdT respectively. siGLO-Green (6-FAM-labeled) was obtained from GE Dharmacon. N-[p-maleimidophenyl] isocyanate (PMPI), and 2-iminothiolane-HCl were obtained form Thermo Scientific. Neurotensin was purchased from Abcam (Cambridge, MA, USA)

Dim N., Perepelyuk M., Gomes O., Thangavel C., Liu Y., Den R., Lakshmikuttyamma A, & Shoyele S.A. (2015). Novel targeted siRNA-loaded hybrid nanoparticles: preparation, characterization and in vitro evaluation. Journal of Nanobiotechnology, 13, 61.

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Immunoglobulin-based PSMA Detection

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Human IgG was purchased from Equitech Bio (TX, U.S.A.). Poloxamer-188, RNase-free water, and fetal bovine albumin (FBS) were obtained from Fisher Scientific. Enzalutamide and monoclonal mouse anti-PSMA from Selleckchem and Millipore were used.

Thangavel C., Perepelyuk M., Boopathi E., Liu Y., Polischak S., Deshpande D.A., Rafiq K., Dicker A.P., Knudsen K.E., Shoyele S.A, & Den R.B. (2018). Improvement in Therapeutic Efficacy and Reduction in Cellular Toxicity: Introduction of a Novel Anti-PSMA-Conjugated Hybrid Antiandrogen Nanoparticle. Molecular pharmaceutics, 15(5), 1778-1790.

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Compound Preparation and Reagents Protocol

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CAPS and AITC (Sigma, St. Louis, MO, USA) were dissolved in dimethyl sulfoxide (DMSO) to obtain a 10 mM stock solution. Further dilutions were made with ECS or Krebs solution to reach final concentrations of 330 and 100 nM in the case of CAPS and 200 or 100 μM in the case of AITC, respectively. RvD1 an RvD2 were purchased from Cayman Chemical in an ethanolic solution. Laurdan was purchased from Sigma and dissolved in (DMSO) to obtain a 10 mM stock solution. PTX was purchased from Sigma and dissolved in ECS. DMEM, horse serum, fetal bovine albumin, and newborn calf serum were purchased from Gibco (Grand Island, NY, USA). Collagenase, deoxyribonuclease I, poly-D-lysine, and NGF were purchased from Sigma.

Payrits M., Horváth Á., Biró-Sütő T., Erostyák J., Makkai G., Sághy É., Pohóczky K., Kecskés A., Kecskés M., Szolcsányi J., Helyes Z, & Szőke É. (2020). Resolvin D1 and D2 Inhibit Transient Receptor Potential Vanilloid 1 and Ankyrin 1 Ion Channel Activation on Sensory Neurons via Lipid Raft Modification. International Journal of Molecular Sciences, 21(14), 5019.

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In-111 Radiolabeling and Characterization

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¹¹¹In was produced in Radiation Application Research School, Karaj, Iran, by ¹¹²Cd (p, 2n) ¹¹¹In reaction. DOTA-NHS was purchased from Macrocyclics (NJ, USA). Fetal Bovine Albumin, RPMI-1640 medium, and L-Glutamine were bought from Gibco Co. (Dublin, Ireland). PD10 De-salting column was inquired from Amersham Pharmacia Biotech; additional chemicals were purchased from Sigma Chemical Co. (MO, USA). Sprague-Dawley rats were obtained from Pasteur Institute (Tehran, Iran). A Bioscan AR-2000 radio thin-layer chromatography (TLC) scanner instrument (Bioscan, Paris, France) was used for Radio-chromatography purposes. A p-type coaxial high-purity germanium (HPGe) detector (model: EGPC 80–200R) coupled with a multichannel analyzer card system and a dose calibrator ISOMED 1010 (Dresden, Germany) were utilized for the measurement of the activity. Calculations were carried out based on the 245 keV peak for ¹¹¹In. The United Kingdom Biological Council's Guidelines on the Use of the Living Animals in Scientific Investigations, 2^nd edition was used to determine the framework of animal experiments. Achieved results are displayed as mean ± standard deviation (mean ± standard deviation), and Student's t-test was used to compare the data based on statistical significance defined as P < 0.05.

Yousefnia H., Zolghadri S, & Alirezapour B. (2022). Human Absorbed Dose Estimation of 111In-DOTA-PR81 as a Novel High Potential Agent for Breast Cancer Imaging. Journal of Medical Physics, 47(2), 194-200.

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