1 thioglycerol mtg

Manufactured by Merck Group

1-thioglycerol MTG is a chemical compound used as a reagent in various laboratory applications. It is a clear, viscous liquid with a characteristic odor. The core function of 1-thioglycerol MTG is to serve as a reducing agent and protective compound in various biochemical and analytical procedures.

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Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin 1x, by Thermo Fisher Scientific (1 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) α mem culture medium, by Thermo Fisher Scientific (1 mentions)

2 protocols using 1 thioglycerol mtg

Mesoderm Differentiation of Stem Cells

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Stem cells were taken off feeder cells with 0.5 mM EDTA (GIBCO) and seeded on gelatine-coated 10 cm plates pre-conditioned with MEF medium over-night and cultured in CDM-PVA supplemented with 12 nM hbFGF (Cell guidance systems, UK) and 10 nM hActivin-A (Source Bioscience, UK). CDM-PVA: 50% IMDM (Invitrogen), 50% advanced DMD-F12 (GIBCO) with 1g/l Poly(vinyl alcohol) PVA (SIGMA), Penicillin/Streptomycin 1x (GIBCO), 1-thioglycerol MTG (SIGMA), Insulin-Transferrin-Selenium 1x (ITS, Life Technologies), Cholesterol 1x (SyntheChol, SIGMA).
As a first step of differentiation, the cells were taken towards the mesoderm germline:

Pance A., Ng B.L., Mwikali K., Koutsourakis M., Agu C., Rouhani F.J., Montandon R., Law F., Ponstingl H, & Rayner J.C. (2023). Novel stem cell technologies are powerful tools to understand the impact of human factors on Plasmodium falciparum malaria. Frontiers in Cellular and Infection Microbiology, 13, 1287355.

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Culturing OP9 Mouse Stromal Cells

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OP9 mouse stromal cells were maintained in the OP9 growth medium, consisting of αMEM culture medium (Invitrogen) containing 100 μM 1-Thioglycerol (MTG) (Sigma-Aldrich), 100 U/ml penicillin/streptomycin and 20% (v/v) FCS. OP9 cells were separated and expanded every 4 days, when the cells were confluent, by trypsinisation. The harvested cells were seeded onto a 10-cm dish coated with 0.1% bovine gelatin solution (Sigma-Aldrich).

Trakarnsanga K., Wilson M.C., Heesom K.J., Andrienko T.N., Srisawat C, & Frayne J. (2018). Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro. Scientific Reports, 8, 1983.

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