TE8 and TE14 cells transfected with a negative control or UTX-siRNA were harvested and homogenized in RIPA (Radio-Immunoprecipitation Assay) lysis buffer, containing protease inhibitor cocktail on ice for 30 min. The homogenate was centrifuged at 4 °C at 15,000 rpm for 30 min, and lysates were collected. Protein concentration was determined using the BCA Protein Assay kit (Thermo Fisher, Waltham, MA, USA). The cell lysates were subjected to the 10% SDS-PAGE gels for following western blotting. The primary antibodies (anti-UTX, anti-E-cadherin, anti-Vimentin, and anti-β-actin) were obtained from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Sigma (St. Louis, MO, USA).
Anti utx
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-UTX is a recombinant antibody that recognizes the UTX (KDM6A) protein. UTX is a histone demethylase that specifically removes the repressive H3K27me3 mark, thereby activating gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Merck Group (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti h3k4me3, by Cell Signaling Technology (1 mentions)
Anti nf ya, by Santa Cruz Biotechnology (1 mentions)
Anti h3k4me1, by Abcam (1 mentions)
Anti actin, by Abcam (1 mentions)
Anti cas9, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti mouse or anti rabbit secondary antibodies, by GE Healthcare (1 mentions)
Anti menin, by Fortis Life Sciences (1 mentions)
Anti h3, by Abcam (1 mentions)
Tween 20, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti hsp90, by BD (1 mentions)
Anti ha, by BioLegend (1 mentions)
2 protocols using anti utx
1
Analyzing UTX Regulation of EMT Markers
2
Western Blot Analysis of Chromatin Regulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole-cell lysates were separated by SDS-PAGE, transferred to a PVDF membrane (EMD Millipore), blocked in 5% nonfat milk in TBS plus 0.5% Tween-20 (Sigma-Aldrich), probed with primary antibodies, and detected with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (GE Healthcare). Primary antibodies used included anti-Cas9 (Cell Signaling Technology, 14697), anti-UTX (Cell Signaling Technology, 33510), anti-Menin (Bethyl, A300-105A), anti-NF-YA (Santa Cruz Biotechnology, sc-17753), anti-Actin (Abcam, ab8224), anti-HSP90 (BD Biosciences, 610418), anti-HA (BioLegend, 901501), anti-H3K4me1 (Abcam, ab8895), anti-H3K4me3 (Cell Signaling Technology, 9751), and anti-H3 (Abcam, ab1791).
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