Lymphocyte separation medium (Ficoll paqué ™) was purchased from Lonza (Basel, Switzerland). RPMI 1640 was purchased from Biochrom (Berlin, Germany) human serum was purchased from Bio Whittaker ( Walkersville, MD), L-glutamine was purchased from Life Technologies (Paisley, Scotland), penicillin, streptomycin, Concanavalin A (Con A) and Brefeldin A was purchased from Sigma (Sigma Chemical Co., Munich, Germany), Interleukin-2 (IL-2) were obtained from R&D Systems (Minneapolis, Minnesota, USA). Phosphate buffer saline (PBS) was obtained from (Verviers, Belgium). CD3 (perCP.Cy5•5), CD3 (APC) clone (RXB-14/IG), CD4 (Allophycocyanin APC-A), clone ( SK3) CD56 (FITC), granzyme B (GzB) Phycoerythrin (PE) clone(RXB-14/IG9), FACS Perm were purchased from (BD Biosciences (BD), San Jose, CA, USA). Sheath Fluid was purchased from (Luminex Corp, Austin, TX, USA).
Interleukin 2 il 2
Manufactured by R&D Systems
Sourced in Germany, United States
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is involved in the proliferation and differentiation of T cells and natural killer cells, which are critical for the body's response to infections and diseases.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Thermo Fisher Scientific (2 mentions)
Facs perm, by BD (1 mentions)
Rpmi 1640, by Harvard Bioscience (1 mentions)
Human serum, by Lonza (1 mentions)
Trypan blue dye, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Concanvalin a cona, by Merck Group (1 mentions)
Cisplatin cis diamminedichloroplatinum 2, by Merck Group (1 mentions)
Rpmi 1640 media, by Harvard Bioscience (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fluorescence activated cell sorting (facs), by BD (1 mentions)
2 protocols using interleukin 2 il 2
1
Ficoll-based Lymphocyte Isolation
2
Cytotoxicity and Apoptosis Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Crystal violet and trypan blue dyes were purchased from Sigma (St. Louis, Mo., USA). Phosphate buffer saline (PBS) was obtained from (Verviers, Belgium). RPMI-1640 media were purchased from Biochrom (Berlin, Germany). L-glutamine, gentamycin and trypsin-EDTA were purchased from Life Technologies (Paisley, Scotland). Penicillin streptomycin, concanvalin A (Con A) and brefeldin A were purchased from Sigma (Sigma Chemical Co., Munich, Germany). Interleukin-2 (IL-2) was obtained from R&D Systems (Minneapolis, Minnesota, USA). Phosphate buffer saline (PBS) was obtained from (Verviers, Belgium). FACS (fluorescence-activated cell sorting) was purchased from (BD Biosciences, San Jose, CA, USA). Sheath fluid was purchased from (Luminex Crop, Austin, TX, USA) and cisplatin (Cis-diammine dichloro platinumII) purchased from Sigma-Aldrich (St Quentin Fallavier, France). The FITC Annexin V apoptosis (Apoptosis Detection Kit II; Cat. No 556570; BD Bioscience, USA).
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