E z n a forensic kit

Manufactured by Omega Bio-Tek

The E.Z.N.A. Forensic Kit is a laboratory equipment product by Omega Bio-Tek designed for the extraction and purification of DNA from various forensic samples. It provides a streamlined process for obtaining high-quality DNA suitable for downstream applications.

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Lab products found in correlation

Abi prism genetic analyzer, by Thermo Fisher Scientific (1 mentions) Abi 3730xl dna analyzer, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Promega (1 mentions) Abi 3730xl analyzer, by Thermo Fisher Scientific (1 mentions) Taq polymerase, by Promega (1 mentions)

2 protocols using e z n a forensic kit

Isolation and Characterization of Fungal DNA

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At the Institut National de la Recherche Agronomique (INRA), DNA was isolated from dried specimens following a CTAB protocol as described by Zhao et al. (2011) . At the Southwest Forestry University, a commercial DNA extraction kit (E.Z.N.A. Forensic Kit, D3591-01, Omega Bio-Tek) was used for DNA extraction. DNA sequences were obtained from three loci: the internal transcribed spacer (ITS), nuclear large ribosomal subunit (nrLSU) and translation elongation factor 1-alpha (tef-1α). Protocols for amplification of ITS and nrLSU regions followed those of White et al. (1990) with some modifications (Zhao et al. 2010 ), by using primers ITS4 and ITS5, LR0R and LR5, respectively. Amplification of the tef-1α region using primers EF1-983F and EF1-1567R (Morehouse et al. 2003 (link)) followed the procedure described as below:
Sequencing was performed on ABI Prism Genetic analyzer (Applied Biosystems) at Beckman Coulter Genomics, England or on ABI 3730 XL DNA analyzer (Applied Biosystems) at Shanghai Majorbio Bio-Pharm Technology Co., Ltd, China. Consensus sequences were assembled by using SeqMan package of Lasergene software v. 7.1 (DNAStar, Madison, WI, USA). All sequences have been deposited in GenBank and their accession numbers are given in Table 1.

Chen J., Callac P., Parra L.A., Karunarathna S.C., He M.Q., Moinard M., De Kesel A., Raspé O., Wisitrassameewong K., Hyde K.D, & Zhao R.L. (2017). Study in Agaricus subgenus Minores and allied clades reveals a new American subgenus and contrasting phylogenetic patterns in Europe and Greater Mekong Subregion. Persoonia : Molecular Phylogeny and Evolution of Fungi, 38, 170-196.

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DNA Extraction and Sequencing from Dried Specimens

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DNA was extracted from dried specimens using a commercial DNA extraction kit (E.Z.N.A. Forensic Kit, D3591-01, Omega BioTek). The polymerase chain reaction (PCR) reactions and sequencing were performed using the primers ITS5 and ITS4 (White et al. 1990). The PCR reaction contained 5 μL PCR GoTaq buffer (5X, Promega), 2.5 μL deoxy-ribonucleotide triphosphate mix (1.2 mmol/L, Eurobio), 0.5 μL bovine serum albumin (10 mg/mL, Promega), 1 μL of each primer (25 mmol/L), 0.2 μL Taq polymerase (5 U/μL, GoTaq Promega), 1 μL DNA template, and ddH₂O up to 25 μL. PCR thermal cycling conditions followed those of Zhao et al. (2011) with some modifications. The programme was 5 min at 95°C; 35 cycles (denaturation 1 min at 94°C, annealing 1.5 min at 52°C, extension 1.5 min at 72°C); 5 min at 72°C for the final extension. The PCR products were examined electrophoretically in an agarose gel stained with ethidium bromide (EB), then sequenced by Biomed Co. Ltd., using an ABI 3730XL Analyzer and ABI BigDye 3.1 Cycle Sequencing Kit.

Xu M.L., Li G.J., Zhou J.L., Bai X.M, & Zhao R.L. (2016). New species of Cystolepiota from China. Mycology, 7(4), 165-170.

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