Rabbit anti tuj1 alexa fluor 488 conjugate

For immunohistochemical analysis, hCOs were fixed with 4% ice-cold paraformaldehyde (PFA) at 4°C for 1 hr. Fixed hCOs were embedded in Optimal Cutting Temperature (OCT) Compound and sectioned at 20 μm. Sections were blocked for 1 hr in PBS containing 0.3% Triton X-100 and 10% normal goat serum. The sections were incubated at 4°C overnight with primary antibodies in blocking solution, as follows: rabbit anti-SOX2 (1:300; Cell Signaling Technology, Danvers, MA), rabbit anti-TUJ1 (Alexa Fluor^®−488 Conjugate; 1:500; MilliporeSigma, Burlington, MA), chicken anti-MAP2 (1:1000; Abcam, Waltham, MA), rabbit anti-GFAP (1:2000; Dako, Santa Clara, CA), rabbit anti-FOXP2 (1:1000; Abcam), rabbit anti-OLIG2 (1:300; MilliporeSigma), chicken anti-MBP (1:2000; ThermoFisher Scientific, Waltham, MA). All secondary antibodies were ThermoFisher Scientific Alexa Fluor^™-conjugated secondary antibodies used at a dilution of 1:500. The sections were imaged with Zeiss Axio Imager.M2.

For immunohistochemical analysis, hCOs were fixed with 4% ice-cold paraformaldehyde at 4 °C for 1 h. Fixed hCOs were embedded in Optimal Cutting Temperature (OCT) Compound and sectioned at 20 μm. Sections were blocked for 1 h in PBS containing 0.3% Triton X-100 and 10% normal goat serum. The sections were incubated at 4 °C overnight with primary antibodies in blocking solution, as follows: rabbit anti-SOX2 (1:300; Cell Signaling Technology, Danvers, MA), rabbit anti-TUJ1 (Alexa Fluor^®-488 Conjugate; 1:500; MilliporeSigma, Burlington, MA), chicken anti-MAP2 (1:1000; Abcam, Waltham, MA), rabbit anti-GFAP (1:2000; Dako, Santa Clara, CA), rabbit anti-FOXP2 (1:1000; Abcam), rabbit anti-OLIG2 (1:300; MilliporeSigma), chicken anti-MBP (1:2000; ThermoFisher Scientific, Waltham, MA). All secondary antibodies were ThermoFisher Scientific Alexa Fluor™-conjugated secondary antibodies used at a dilution of 1:500. The sections were imaged with Zeiss Axio Imager.M2.