HepG2 cells (HB-8065) were purchased from ATCC (Manassas VI, USA) and iPS(IMR90)-4 cells were purchased from WiCell Research Institute (Madison WI, USA). In all experiments, HepG2 cells were cultured in RPMI 1640 (Thermo Fisher Scientific, USA) supplemented with 10 % (v/v) fetal bovine serum (FBS, RPMI, Thermo Fisher Scientific, USA). iPS(IMR90)-4 were cultured in Essential 8 medium (E8, Thermo Fisher Scientific, USA). Henceforth, these will be referred to as culture medium. Cells were cultured under standard tissue culture conditions (37 °C, 5 % CO2, 95 % relative humidity).
Hepg2 cells hb 8065
Manufactured by American Type Culture Collection
Sourced in United States
HepG2 cells (HB-8065) are a well-characterized human hepatocellular carcinoma cell line derived from the liver tissue of a 15-year-old Caucasian male. The cells exhibit epithelial-like morphology and are commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Penicillin g, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
L glutamine, by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Amphotericin b, by Merck Group (2 mentions)
Lx 2 cells, by Merck Group (1 mentions)
Ham s f 12 nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Non essential amino acids (neaa), by Fujifilm (1 mentions)
Penicillin streptomycin, by Fujifilm (1 mentions)
Pyruvate, by Fujifilm (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Crl 2254, by American Type Culture Collection (1 mentions)
Medium 199, by Thermo Fisher Scientific (1 mentions)
Hepg2 cell, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
17 protocols using hepg2 cells hb 8065
1
Culturing HepG2 and iPS cells
2
Investigating Aroclor 1260 Effects on Liver Cells
3
Generation of 4CYPs-POR Artificial Chromosome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A MAC6 vector was used to generate the 4CYPs-POR MAC. The structure of MAC6 contained a centromere from mouse chromosome 11, EGFP flanked by HS4 insulators, PGKneo, loxP-5’HPRT site, PGKpuro, and telomeres (S2 Fig ) [27 (link)]. Hypoxanthine phosphoribosyl transferase (HPRT)-deficient Chinese hamster ovary (CHO; JCRB0218) hybrids containing only the MAC6 or the 4CYPs-POR MAC were maintained in Ham’s F-12 nutrient mixture (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum and 600 μg/mL G418. HepG2 cells (HB-8065, ATCC, USA) were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, non-essential amino acids (Wako), pyruvate (Wako), and penicillin-streptomycin (Wako).
4
Culturing Hepatocellular Carcinoma Cell Lines
5
Culturing HepG2 Cells in EMEM Media
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2 cells (HB-8065) were bought from the ATCC®, cells were cultured at 37 °C and 5% CO 2 in EMEM medium with several other ingredients including FBS, Lglutamine, HEPES, amphotericin B, penicillin G, and streptomycin with a concentration of 10% v/v, 2 mM, 20 mM, 25 ng/mL, 100 IU/mL, 0.1 mg/mL, respectively..
6
Hepatocellular Carcinoma Patient Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2 cells (HB-8065, ATCC, Manassas, VA) were maintained in Dulbecco's modified Eagle medium (Invitrogen, Carlsbad, CA) supplemented with 10% (v/v) fetal bovine serum (Invitrogen). Surgically resected adjacent normal liver tissues were obtained from 24 hepatocellular carcinoma (HCC) patients who underwent surgical resection. All samples were immediately frozen and stored in liquid nitrogen. The study protocol had all the appropriate approvals by the institutional review board and regulatory authorities. All patients had given informed and written consent.
7
Culturing Human Hepatocellular Carcinoma and Murine Hepatocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human hepatocellular carcinoma HepG2 cells (HB-8065) and normal murine hepatocyte AML12 (alpha mouse liver 12) cells (CRL-2254) were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA). HepG2 cells were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. AML12 cells were grown in a mixture of DMEM and Ham's F12 medium supplemented with 0.005 mg/ml insulin, 0.005 mg/ml transferrin, 5 ng/ml selenium, 40 ng/ml dexamethasone, and 10% FBS. All cells were incubated in humidified atmosphere containing 5% CO2 and 95% air at 37°C.
8
Cell Culture of Human Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human mesothelial MeT-5A cells (CRL-9444) and HepG2 cells (HB-8065) were purchased from the American Type Culture Collection. Human mesothelial MES-F cells were from Zen-Bio Inc., and human liver myofibroblast cell line LX-2 cells were donated from Professor Scott Friedman at Mount Sinai School of Medicine. All cell lines were maintained at 37°C and 5% CO2 in a humidified atmosphere. MeT-5A cells and MES-F cells were cultured in Medium 199 (Gibco) with 10% FBS (Thermo Scientific). HepG2 cells were cultured in RPMI 1640 (Gibco) with 10% FBS. LX-2 cells were cultured in DMEM (Gibco) with 10% FBS.
9
Liposomal Transfection Reagent Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DLin-MC3-DMA was purchased from MedKoo Biosciences. DSPC, DOPE, DOTAP, DDAB, 18PG (sodium salt) and 14PA (sodium salt) were purchased from Avanti Polar Lipids. Cholesterol was purchased from Sigma. DMG-PEG (MW 2000) (DMG-PEG2000) was purchased from NOF America Corporation. HepG2 cells (HB-8065™) and B16F10 cells (CRL-6475) were purchased from (American Type Culture Collection, USA). Reporter lysis buffer and luciferin assay solution were purchased from Promega. All pDNA was purchased from Aldevron. All siRNA was purchased from ThermoFisher.(STAT1 siRNA (Cat# AM16708), NFkβ2 siRNA (Cat# AM16708)) d -Luciferin (sodium salt) was purchased from Gold Biotechnology.
10
Hypoxia Exposure on HepG2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All cell culture media and reagents were purchased from Gibco, Grand Is., NY, USA. The human hepatoma cell line HepG2 cells (HB 8065), purchased from American Type Culture Collection, Rockville, MD, USA, were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum in a humidified 5% CO2 incubator (Forma 3111, Thermo, Marietta, Georgia, USA) at 37oC. The cells were placed in Hank's medium without glucose, and then exposed to hypoxia (94% N2, 5% CO2, and 1% O2) for 4, 8, 12, or 24 hours at 37oC in an incubator (Ruskin, Biotrace, Bridgend, UK) as previously described 32 -33 (link). Control cultures were exposed to normoxia only. In some experiments, the cells were pre-treated with 20 mMH-89 or 20 mM ofLY294002, and then incubated in DMEM medium containing 10% serum for 1 hour before hypoxia.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!