Kb cells

Manufactured by American Type Culture Collection

Sourced in United States

KB cells are a type of epithelial cell line derived from human oral carcinoma. They are commonly used in cell culture research and assays. The core function of KB cells is to serve as a model system for studying various cellular processes and responses.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Lysotracker green, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Sn 38, by Merck Group (1 mentions) A549 cell, by American Type Culture Collection (1 mentions) Cbdca, by Nippon Kayaku (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Gibco rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Poly l glutamic acid sodium salt, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Ambisome, by Gilead Sciences (1 mentions)

Spelling variants of this product

KB cells (ATCC CCL-17) (4 citations) KB-3-1 cell line (2 citations)

10 protocols using kb cells

Culturing Human Oral Squamous Carcinoma Cells

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The human oral squamous carcinoma KB cells were from ATCC (Manassas, VA, USA). The cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin at 37°C in one atmosphere with 5% CO₂.

Tseng P.Y., Lu W.C., Hsieh M.J., Chien S.Y, & Chen M.K. (2014). Tanshinone IIA Induces Apoptosis in Human Oral Cancer KB Cells through a Mitochondria-Dependent Pathway. BioMed Research International, 2014, 540516.

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Lysosomal Targeting Probes Evaluation

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Human dermal fibroblasts, HeLa, and KB cells were purchased from ATCC (Manassas, VA). The cells grown for a minimum of five passages were used in all experiments. The cells were seeded at a density of 10 000 cells/cm² on glass cover slides that were placed in 12-well culture plates and maintained in Dulbecco's modified eagle medium (DMEM, Thermo-Fisher, Waltham, MA) with 20% fetal bovine serum (FBS, Atlanta Biologicals, Flowery Branch, GA). After 16 h of incubation, the cell culture medium was replaced by freshly prepared serum-free medium with 2, 5, 10, 15, and 20 μM of probe A or B. The cells were incubated further with 50 nM LysoTracker Green (Thermo-Fisher) for 30 min, and 1 mg/mL Hoechst for 5 min in order to confirm the specific target of our probes to lysosomes in cells. Live cell images were taken by a confocal fluorescence microscope. LysoTracker Green fluorescence, CF and FUCL images were taken in the same view field by appropriate excitation and emission to simultaneously visualize the extent of localization of our probes and LysoTracker Green in the same intracellular compartment. The exposure time for each filter was kept constant. The colocalization analysis based on Pearson's coefficient was obtained by the JACoP plugin from ImageJ.^{40 (link)}

Zhang S., Chen T.H., Lee H.M., Bi J., Ghosh A., Fang M., Qian Z., Xie F., Ainsley J., Christov C., Luo F.T., Zhao F, & Liu H. (2017). Luminescent Probes for Sensitive Detection of pH Changes in Live Cells through Two Near-Infrared Luminescence Channels. ACS sensors, 2(7), 924-931.

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Xenograft Model of Nasopharyngeal Carcinoma

Cited 1 time

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KB cells (human nasopharyngeal carcinoma cell line, CCL-17; known for overexpressing FRs19 (link),20 (link) were obtained from the American Type Culture Collection (ATCC, VA, USA). Cells were grown in monolayers in FFRPMI culture medium (modified RPMI without folic acid, vitamin B₁₂, and phenol red) in a humidified atmosphere at 37°C containing 5% CO₂. The media was supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyrovate, nonessential amino acids, 100 U/mL penicillin, and 100 µg/mL streptomycin.
Six-week-old female, athymic nude mice (NMRI nu/nu, Taconic Europe, Borup, Denmark) were allowed to acclimatize for 1 week before subcutaneous inoculation on both flanks with KB cancer cells (1.5×10⁶ cells in 100 µL serum-free media and Matrigel).

Christensen E., Henriksen J.R., Jørgensen J.T., Amitay Y., Shmeeda H., Gabizon A.A., Kjær A., Andresen T.L, & Hansen A.E. (2018). Folate receptor targeting of radiolabeled liposomes reduces intratumoral liposome accumulation in human KB carcinoma xenografts. International Journal of Nanomedicine, 13, 7647-7656.

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Establishment of CDDP-resistant Cancer Cell Lines

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KB cells, a human nasopharyngeal carcinoma cell line, and A549 cells, a human lung carcinoma cell line, were obtained from the American Type Culture Collection. CDDP-resistant KB cells, KB/CDDP(T), were established by stepwise dose escalation with CDDP in our laboratory. ECyd was synthesized at Taiho Pharmaceutical Co., Ltd. (Tokyo, Japan). CDDP and CBDCA were obtained from Nippon Kayaku Co., Ltd. (Tokyo, Japan), SN-38 was obtained from Sigma-Aldrich Co., LLC. (Missouri, USA), and ADM was obtained from Kyowa Hakkou Kirin Co., Ltd. (Tokyo, Japan).

Fukushima H., Abe T., Sakamoto K., Tsujimoto H., Mizuarai S, & Oie S. (2014). 3'-Ethynylcytidine, an RNA polymerase inhibitor, combined with cisplatin exhibits a potent synergistic growth-inhibitory effect via Vaults dysfunction. BMC Cancer, 14, 562.

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Culturing Human Oral Carcinoma Cells

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KB cells (human oral carcinoma cells) were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA) and grown continuously as a monolayer in 75 cm² T flasks in GIBCO RPMI 1640 medium (Invitrogen Corporation, Carlsbad, CA, USA) in a humidified incubator at 37 °C and 5% CO₂. RPMI was supplemented with penicillin (100 units/ml), streptomycin, and 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen Corporation, Carlsbad, CA, USA) before use.

Pearson R.M., Sen S., Hsu H.J., Pasko M., Gaske M., Král P, & Hong S. (2016). Tuning the Selectivity of Dendron Micelles through Variations of the Poly(ethylene glycol) Corona. ACS nano, 10(7), 6905-6914.

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Antifungal Nanoparticle Evaluation Protocol

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Poly-l-glutamic acid sodium salt (20–40 kDa and 50–70 kDa; catalog numbers G0546 and G0421, respectively), AmB (European Pharmacopoeia reference standard), polyvinyl alcohol (PVA), Roswell Park Memorial Institute (RPMI) 1640 medium, and dimethyl sulfoxide were purchased from Sigma Aldrich (St Louis, MO, USA). The antifungal agents AmBisome (Gilead Sciences Inc, San Dimas, CA, USA) and Fungizone (Apothecon, Princeton, NJ, USA) were reconstituted according to the manufacturers’ instructions. KB cells (HeLa contaminant, cervical adenocarcinoma-derived epithelial cells) and mouse leukemia RAW 264.7 cells (macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice) were sourced from the American Type Culture Collection (ATCC, Manassas, VA, USA). Detection kits for liver and kidney function test parameters were purchased from Span Diagnostics Ltd (Mumbai, India). The rest of the chemicals were of analytical grade of purity and sourced locally.

Zia Q., Khan A.A., Swaleha Z, & Owais M. (2015). Self-assembled amphotericin B-loaded polyglutamic acid nanoparticles: preparation, characterization and in vitro potential against Candida albicans. International Journal of Nanomedicine, 10, 1769-1790.

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Mithramycin treatment of HEp-2 and KB cells

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HEp-2 cells were from Kyungpook National University (Daegu, Korea) and KB cells were from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in DMEM 100 U/mL each of penicillin and streptomycin and 10% FBS for HEp-2 cells and 5% FBS for KB in a humidified atmosphere containing 5% CO₂ at 37°C. Equal numbers of cells were seeded and allowed to attach. At 50–60% confluence, cells were treated with DMSO or indicated concentrations of Mith diluted in DMEM with 5% FBS for HEp-2 cells and 2.5% for KB cells. Mith was dissolved in 0.1% DMSO.

Choi E.S., Nam J.S., Jung J.Y., Cho N.P, & Cho S.D. (2014). Modulation of specificity protein 1 by mithramycin A as a novel therapeutic strategy for cervical cancer. Scientific Reports, 4, 7162.

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Culturing Human Oral and Rat Cancer Cells

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Human oral epidermoid carcinoma KB cells obtained from American Type Culture Collection (ATCC) were grown in RPMI medium supplemented with 10% FCS. MC28 cells, a methylcholanthrene-induced rat fibrosarcoma cell line, were grown in DMEM supplemented with 10% FCS at 37 °C in a humidified atmosphere containing 5% CO₂. Unless otherwise stated materials for the cell studies were purchased from Sigma-Aldrich (Gillingham, UK).

Yaghini E., Dondi R., Tewari K.M., Loizidou M., Eggleston I.M, & MacRobert A.J. (2017). Endolysosomal targeting of a clinical chlorin photosensitiser for light-triggered delivery of nano-sized medicines. Scientific Reports, 7, 6059.

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Culturing Human Oral Squamous Carcinoma Cells

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The human oral squamous carcinoma cancer (KB) cells were purchased from the American Type Culture Collection (Manassas, VA, USA), cells were cultured in RPMI-1640 supplemented with 10% FBS, and incubated at 37°C with 5% CO₂ and 95% humidity.

Xie W., Zhang Z., Song L., Huang C., Guo Z., Hu X., Bi S, & Yu R. (2018). Cordyceps militaris Fraction induces apoptosis and G2/M Arrest via c-Jun N-Terminal kinase signaling pathway in oral squamous carcinoma KB Cells. Pharmacognosy Magazine, 14(53), 116-123.

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KB Cell Culture for Nasopharyngeal Cancer

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KB cells (nasopharyngeal epidermal carcinoma) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and used between passages 18–24. The cells were cultured in RPMI-1640 medium supplemented with 10% (v/v) FBS and 1% penicillin streptomycin at 37 °C in a saturated humidity of 5% CO₂. KB cells were seeded at a density of 2 × 10⁵ cells/well and were cultured for 24 h before incubation with THPE-NCs.

Zhang J., Corpstein C.D, & Li T. (2020). Intracellular uptake of nanocrystals: Probing with aggregation-induced emission of fluorescence and kinetic modeling. Acta Pharmaceutica Sinica. B, 11(4), 1021-1029.

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