The cell lines HEK293-F (Gibco; RRID:CVCL_D603), B16F10 (ATCC Cat# CRL-6475, RRID:CVCL_0159), CTLL-2 (ATCC Cat# TIB-214, RRID:CVCL_0227), and HEK Blue IL-12 (Invivogen; RRID:CVCL_UF31) were cultured according to vendor instructions. All cells were maintained in 5% CO2 at 37°C and tested negative for mycoplasma. B16F10 cells were confirmed to be negative for rodent pathogens (IMPACT I test, IDEXX) prior to murine tumor studies. Six- to eight-week old female C57BL/6 mice (Taconic) were purchased and used for murine Nanostring and combination therapy efficacy experiments. All mouse studies were conducted under approval of the MIT Committee on Animal Care in accordance with federal, state, and local guidelines. Additional details on study designs and dosing can be found in the Supplementary Methods S1 .
Impact 1 test
Manufactured by IDEXX
Sourced in United States
The IMPACT I test is a diagnostic tool designed to quickly and accurately measure blood parameters in animals. It provides veterinary professionals with essential information to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Hek293f, by Thermo Fisher Scientific (1 mentions)
Ctll 2, by American Type Culture Collection (1 mentions)
Hek blue il 12, by InvivoGen (1 mentions)
C57bl 6 mice, by Taconic Biosciences (1 mentions)
Flag m2, by Merck Group (1 mentions)
Anti actin, by Merck Group (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Gm6001, by Merck Group (1 mentions)
Anti vinculin, by Merck Group (1 mentions)
Anti gfp, by Merck Group (1 mentions)
Vitronectin, by Merck Group (1 mentions)
Inositol, by Merck Group (1 mentions)
β actin ac 15, by Merck Group (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
B cereus pi plc, by Thermo Fisher Scientific (1 mentions)
2 protocols using impact 1 test
1
Cell Line Maintenance and Mouse Studies
2
Cell Culture and Antibody Validation for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HEK293, MDA-MB-231 and Hs578T cells were obtained from the ATCC and grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37°C under 5% CO2. Original MDA-MB-231 cells were pathogen tested using the ImpactI test (Idexx Bioresearch, Westbrook, ME, USA) and were negative for all pathogens tested. All cell lines were routinely tested negative for mycoplasma contamination. Antibodies used: anti-mCh and anti-GFP, home-made; anti-Flag, M2, anti-Vinculin and β-Actin (AC-15) from Sigma; anti-uPAR (MAB807) from R&D systems; anti-uPAR (13F6) (Zhao et al., 2015 (link)); anti-FAK(pTyr397) from Thermo Fisher. Vitronectin, fibronectin, inositol 1-phosphate (dipotassium salt) and inositol were purchased from Sigma-Aldrich. B. cereus PI-PLC was from Molecular Probes. Phalloidin red (actin-stain 647 phalloidin) and green (actin-stain 488 phalloidin) were from Cytoskeleton. GM6001 was from Millipore. Research Source Identifiers: MDA-MB-231 cells RRID:CVCL_0062 ; Hs578T cells RRID:CVCL_0332 ; Antibodies: Flag M2 RRID:AB_259529 ; Anti Vinculin RRID:AB_10746313 ; Anti actin RRID:AB_2223210 ; uPAR RRID:AB_2165463 .
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