C57BL mice (17 ± 1.5 g) (n = 40) were obtained from the Experimental Animal Center, Fuzhou Medical College, Nanchang University, Fuzhou, Jiangxi, China. The animals were maintained at a controlled temperature (22°C ± 1°C), humidity (50%), and light (12/12-h light/dark). All animal experiments were approved by the Experimental Animal Ethics Committee of Fuzhou Medical College, Nanchang University. Inulin was obtained from MCE (HY-N7075) and dissolved in drinking water (10% w/w). The blood glucose testing equipment was offered by Sinocare. The TG, low-density lipoprotein (LDL), high-density lipoprotein, cholesterol, free fatty acids, interleukin-6, interleukin-1β, and tumor necrosis factor testing kits were obtained from Nanjing Jiancheng Company. The insulin ELISA kit was purchased from Abcam. The HFD consisted of 20% carbohydrates, 35% proteins, and 45% fats (D12492; Research Diets; 60% of total calories).
Interleukin 1β
Manufactured by Nanjing Jiancheng
Interleukin-1β is a cytokine that plays a crucial role in the regulation of immune and inflammatory responses. It is involved in the activation and differentiation of various immune cells, such as T cells, B cells, and macrophages. Interleukin-1β is a key mediator of the inflammatory response and is involved in the pathogenesis of various inflammatory and autoimmune diseases.
Automatically generated - may contain errors
Lab products found in correlation
D12492, by Research Diets (1 mentions)
Insulin elisa kit, by Abcam (1 mentions)
Free fatty acids, by Nanjing Jiancheng (1 mentions)
Interleukin 6 (il 6), by Nanjing Jiancheng (1 mentions)
Gsk 3β, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated goat anti mouse igg, by Merck Group (1 mentions)
Anti rabbit igg, by Merck Group (1 mentions)
Caspase 3, by Nanjing Jiancheng (1 mentions)
Malondialdehyde, by Nanjing Jiancheng (1 mentions)
Glutathione (gsh), by Nanjing Jiancheng (1 mentions)
2 protocols using interleukin 1β
1
Inulin Supplementation in HFD-Fed Mice
2
Antibodies for NF-κB, GSK3β, and Inflammation
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Rabbit monoclonal antibodies against acetyl-NF-κB p65 (catalog # PA5-17264), GSK3β (catalog # 710132), and mouse monoclonal antibody against Lamin B and β-actin (catalog # MA1-140) were procured from Thermo Fisher Scientific (Waltham, MA, USA). Anti-rabbit IgG (catalog # A0545), horseradish peroxidase-conjugated goat anti-mouse IgG, and secondary antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Interleukin-6 kit (catalog # H007), interleukin-1β (catalog # H002), glutathione (catalog # A006-2), malondialdehyde (catalog # A003-1), and caspase-3 (catalog # H076) were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, People’s Republic of China).
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