Fruits of Berberis integerrima were collected from Kohmar (Fars Province, Iran) during August and September 2105. The voucher numbers for fruits was P.M. 396. They were deposited at Museum of Medicinal Plants, Shiraz University of Medical Sciences, Shiraz, Iran.
The fruits were freeze-dried using freeze dryer (Zirbus, Germany) and ground into powder. The powder (100 g) was macerated with ethanol (70%, 1 L) in a dark place, followed by filtration. The extract was concentrated using a rotary evaporator (Heidolph, Germany) under vacuum, followed by speed vacuum to get 60 g gummy material. The crude extract (30 g) was suspended in trifluoroacetic acid (TFA) (Samchun, Korea) (0.3%), was filtered and poured into the decanter, where it was properly shaken with ethyl acetate (Samchun, Korea). This extraction procedure was repeated three times and the aqueous phase was collected in each repeat. The aqueous phase was loaded on Amberlite column XAD-7 (2.5 × 45 cm) (Sigma-Aldrich, St Louis, MO, USA) as the stationary phase. This phase was rinsed with distilled water containing TFA, respectively. At the last time, the stationary phase was rinsed with methanol containing TFA, and then the extract was collected and concentrated using a rotary evaporator. Yield of extraction fractionation was 20%.
The fruits were freeze-dried using freeze dryer (Zirbus, Germany) and ground into powder. The powder (100 g) was macerated with ethanol (70%, 1 L) in a dark place, followed by filtration. The extract was concentrated using a rotary evaporator (Heidolph, Germany) under vacuum, followed by speed vacuum to get 60 g gummy material. The crude extract (30 g) was suspended in trifluoroacetic acid (TFA) (Samchun, Korea) (0.3%), was filtered and poured into the decanter, where it was properly shaken with ethyl acetate (Samchun, Korea). This extraction procedure was repeated three times and the aqueous phase was collected in each repeat. The aqueous phase was loaded on Amberlite column XAD-7 (2.5 × 45 cm) (Sigma-Aldrich, St Louis, MO, USA) as the stationary phase. This phase was rinsed with distilled water containing TFA, respectively. At the last time, the stationary phase was rinsed with methanol containing TFA, and then the extract was collected and concentrated using a rotary evaporator. Yield of extraction fractionation was 20%.