Apc tcrγδ

Manufactured by BioLegend

APC-TCRγδ is a flow cytometry antibody reagent that binds to the T cell receptor (TCR) expressed on γδ T cells. It is designed to identify and quantify the population of γδ T cells in a sample.

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2 protocols using apc tcrγδ

Comprehensive Immunophenotyping Panel

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Alexa Fluor 488 NK1.1 (PK136), Biotin-CD4 (GK1.5), Alexa Fluor 488-CD4 (GK1.5), APC-eFluor 780-CD4 (GK1.5), FITC-CD3ε (17A2), Alexa Fluor 647-CD3ε (17A2), Alexa Fluor 488-CD206, Brilliant Violet 421-CD3ε (17A2), FITC-F4/80, Alexa Fluor 488-CD3ε (145-2C11), Alexa Fluor 647 CD49b (DX5), Pacific Blue-CD49b (DX5), PE-NK1.1 (PK136), Brilliant Violet 421 NK1.1 (PK136), PE/Cy5-CD4 (GK1.5), APC-TCRγδ (GL3), Biotin-IFN-γRα (2E2), Brilliant Violet 421-CD25 (PC61), PE-CD25 (PC61), Biotin-CD122 (5H4), PE/Cy7-IFN-γ (XMG1.2), PE-GM-CSF (MP1-22E9), Pacific Blue-TNF-α (MP6-XT22), PercCP/Cy5.5-CD69, Biotin-BrdU (Bu20a), FITC-Ki67 (16A8), Alexa Fluor 647-streptavidin, APC-Cy7-Streptavidin, PE-Cy7-Streptavidin, Brilliant violet 421-CD62L (MEL-14) and rabbit polyclonal anti-Asialo-GM1 (aGM) antibodies were purchased from Biolegend (San Diego, CA). APC-RORγt (AFKJS-9), APC-T-bet (4B10) and PE/Cy7-T-bet (4B10) were procured from eBioscience (San Diego, CA). Anti-NK1.1 (PK136), anti-F4/80 (Cl:A3–1), anti-Gr1 (RB6-8C5) and isotype control antibodies for in vivo use were procured from BioXcell (West Lebanon, NH). Alexa Fluor 647-labeled CD1d tetramer (mCD1d, PBS-57) and unloaded control tetramer were received from NIH Tetramer Core Facility (Atlanta, GA). 5-Bromo-2′-deoxyuridine (BrdU) was purchased from Sigma-Aldrich.

Paul S., Chhatar S., Mishra A, & Lal G. (2019). Natural killer T cell activation increases iNOS+CD206- M1 macrophage and controls the growth of solid tumor. Journal for Immunotherapy of Cancer, 7, 208.

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Immune Cell Profiling in Post-Stroke Mice

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Immune cells were isolated from the ileum, cecum and colon of post-stroke and sham mice and analyzed by flow cytometry.⁹¹ (link) Dead cells were detected using LIVE/DEAD® Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific). Cell surface and intracellular markers were stained with following fluorescent antibodies: Super Bright 645-CD45 (catalog 64-0451-82) from Invitrogen and APC/Cy7-CD3 (catalog 100330), Brilliant Violet 605-CD4 (catalog 100548), PerCP/Cy5.5-CD8 (catalog 100734), APC-TCR γ/δ (catalog 118116), PE-IL-17A (catalog 506904), PE/Cy7-IFNγ, FITC-IL-10 (catalog 505006), eFluor450-FoxP3 (catalog 48-5773-82) from BioLegend. Data were collected by a Cytek Aurora flow cytometer and analyzed with FlowJo software (v10.8.1). After dead and doublet cell exclusion and CD45⁺ live cell selection, γδ T cells were defined as CD3⁺ CD4⁻ TCR γ/δ⁺ and CD4 T cells as CD3⁺ CD8⁻ CD4⁺.

Ge Y., Zadeh M., Yang C., Candelario-Jalil E, & Mohamadzadeh M. (2022). Ischemic Stroke Impacts the Gut Microbiome, Ileal Epithelial and Immune Homeostasis. iScience, 25(11), 105437.

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