Tc100

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

The TC100 is a thermal cycler designed for DNA amplification and other temperature-sensitive reactions. It features a compact and user-friendly design, with a built-in touchscreen interface for easy program setup and monitoring. The TC100 can accommodate a range of sample volumes and tube formats, making it a versatile tool for various laboratory applications.

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Lab products found in correlation

3 protocols using tc100

Cell Culture Reagents and Antibodies

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Cell culture media RPMI1640, DMEM, TC100 and supplements (Newborn calf serum, penicillin/streptomycin, glutamine, trypsin-EDTA) were from Gibco Life Technologies (Paisley, UK). NCTC 109 medium and chloroquine were from Sigma (Steinheim, Germany). Foetal bovine serum (FBS) was from Lonza Inc. (Walkersville, MD, USA). Lipofectamine 2000 and Lysotracker Red (LTR) were from Life Technologies (Carlsbad, CA, USA). U18666A was from Merck4Biosciences (Madrid, Spain). Anti-GFP was from Roche Diagnostics GmbH (Mannheim, Germany). Anti-Hsp90 (sc-7947), anti-RhoGDI (sc-360), and anti-vimentin (sc-6260) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse Igs were from Dako (Copenhagen, Denmark). Anti-giantin was from Covance (Princeton, NJ, USA).

Oeste C.L., Pinar M., Schink K.O., Martínez-Turrión J., Stenmark H., Peñalva M.A, & Pérez-Sala D. (2014). An Isoprenylation and Palmitoylation Motif Promotes Intraluminal Vesicle Delivery of Proteins in Cells from Distant Species. PLoS ONE, 9(9), e107190.

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Insect Cell Culture Protocol

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Insect cell lines Sf9 and Sf21 from Spodoptera frugiperda and TnHi5 from Trichoplusia ni, were cultured at 28 °C. Sf9 and TnHi5 cells were maintained in ESF 921 serum-free medium (Expression Systems) and Sf21 cells were maintained in TC100 (Gibco) medium supplemented with 10% (v/v) foetal bovine serum (FBS) [27] .

Aksular M., Calvo-Pinilla E., Marín-López A., Ortego J., Chambers A.C., King L.A, & Castillo-Olivares J. (2018). A single dose of African horse sickness virus (AHSV) VP2 based vaccines provides complete clinical protection in a mouse model. Vaccine, 36(46), 7003-7010.

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Culturing Insect and Kidney Cells

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Insect cells were maintained at 28°C in Erlermeyer flasks (Corning) and glass Spinner flasks, respectively. Sf-9 cells were passaged before reaching their stationary phase using Sf9-S2 medium (PAA Laboratories Ltd); Sf-21 cells were maintained in TC100 (GIBCO, Life Technologies Ltd) supplemented with 10% FBS. HK-2 cells were purchased from American Type Culture Collection (ATCC) and cultured in Keratinocyte Serum Free Medium (KSFM; GIBCO, Life Technologies Ltd.) supplemented with 2% antibiotics (100 IU/ml penicillin, 100 µg/ml streptomycin) at 37°C 5% CO 2 .

Hitchman E., Hitchman R.B, & King L.A. (2017). BacMam Delivery of a Protective Gene to Reduce Renal Ischemia-Reperfusion Injury. Human gene therapy, 28(9).

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