35 m filter

Manufactured by BD

The 35 µm filter is a laboratory equipment designed to remove particles or contaminants from a liquid or gas stream. It has a pore size of 35 microns, which allows the passage of the fluid while trapping larger particles. The primary function of this filter is to provide a simple and effective way to purify or clarify a sample prior to further analysis or processing.

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Lab products found in correlation

Facsdiva software, by BD (2 mentions) Fc block, by BD (2 mentions) Pharm lyse, by BD (2 mentions) Countess 2, by Thermo Fisher Scientific (2 mentions) Genomics chromium, by 10x Genomics (2 mentions) Human fgf basic, by Thermo Fisher Scientific (2 mentions) Countess, by Thermo Fisher Scientific (2 mentions) Dmem f 12 glutamax, by Thermo Fisher Scientific (2 mentions) Sh800, by Sony (2 mentions) Cd45 apc, by R&D Systems (1 mentions) Ly6g pe, by R&D Systems (1 mentions) Ly6c percpcy5, by R&D Systems (1 mentions) Live dead fixable blue dead cell stain, by Thermo Fisher Scientific (1 mentions) 1xb27 ra, by Thermo Fisher Scientific (1 mentions)

4 protocols using 35 m filter

Characterizing Leukocyte Infiltrates in S. aureus Biofilm Infection

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To characterize leukocyte infiltrates in inflamed soft tissues
surrounding the knee joint during S. aureus biofilm infection,
tissues were excised, dissociated using the rubber end of a plunger from a 3 cc
syringe, and passed through a 35 µm filter (BD Falcon, Bedford, MA). The
resulting filtrate was washed with 1× PBS and cells were collected by
centrifugation (300 × g, 10 min), whereupon RBCs were
lysed using BD Pharm Lyse (BD Biosciences; San Diego, CA). After lysis, cells
were resuspended in PBS containing 2% FBS, followed by incubation in Fc
Block (BD Biosciences, San Diego, CA) to minimize non-specific antibody binding.
Cells were stained with CD45-APC, Ly6G-PE, Ly6C-PerCPCy5.5, F4/80-PE Cy7,
CCR2-FITC (R&D Systems; Minneapolis, MN), and CD11b-eFluor450. All
fluorochrome-conjugated antibodies were purchased from BD Biosciences (San
Diego, CA) or eBioscience (San Diego, CA) unless otherwise indicated. An aliquot
of cells was stained with isotype-matched control antibodies to assess the
degree of non-specific staining and fluorescence minus one was used to identify
gating thresholds (22 (link)). The number of
events analyzed ranged from 20,000–100,000 per sample, depending on the
experimental setup. Analysis was performed using BD FACSDiva software with cells
gated on the total leukocyte population (CD45⁺).

Heim C.E., Vidlak D., Scherr T.D., Kozel J.A., Holzapfel M., Muirhead D.E, & Kielian T. (2014). Myeloid-derived suppressor cells (MDSCs) contribute to S. aureus orthopedic biofilm infection. Journal of immunology (Baltimore, Md. : 1950), 192(8), 3778-3792.

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Isolation and Characterization of Hes1+ Neural Stem Cells

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To isolate Hes1 positive neural stem cells (NSCs) at E14, dorsal cortices were dissected from Hes1-d2-EGFP^Tg/+ mice. Cortices were dissociated with 0.05% trypsin with Hanks’ balanced salt solution (HBSS) (–) at 37 °C for 10 min. After centrifugation at 1000×g for 5 min, cells were resuspended with 0.375% BSA/HBSS(−) by gentle pipetting 15 to 20 times. Resuspended cells were filtered with 35-µm filter (Falcon) and sorted into sorting buffer (20 ng/ml human basic FGF (Peprotech), 1×B27 RA- (Gibco), in Dulbecco’s Modified Eagle Medium (DMEM) F12 + GlutaMax (Gibco)) by a cell sorter (SH800, SONY) equipped with 130-µm sorting chips (SONY, LE-C3113). After sorting, cell number was counted by Countess or Countess II (Invitrogen). For cells from wild-type and Fbl-mutant mice, cells were collected similarly except that sorting was not performed.
Collected cells were immediately loaded into the 10X-Genomics Chromium (10X Genomics, Pleasanton, CA). Libraries for single-cell cDNA were prepared using Chromium 3′ v2 platform as the manufacturer′s protocol.

Wu Q., Shichino Y., Abe T., Suetsugu T., Omori A., Kiyonari H., Iwasaki S, & Matsuzaki F. (2022). Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells. Nature Communications, 13, 470.

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Characterizing Macrophage Infiltrates in Knee Biofilm Infection

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To characterize macrophage infiltrates in inflamed soft tissues surrounding the knee joint during S. aureus biofilm infection, tissues were excised, dissociated with the rubber end of a plunger from a 3-ml syringe, and passed through a 35-µm filter (BD Falcon, Bedford, MA). The resulting filtrate was washed with 1× PBS, and cells were collected by centrifugation (300 × g, 10 min), whereupon red blood cells were lysed with BD Pharm Lyse (BD Biosciences, San Diego, CA), resuspended in 1× PBS, and incubated in Fc Block (BD Biosciences) to minimize nonspecific Ab binding. Cells were stained with CD45-allophycocyanin, Ly6G-phycoerythrin (PE), Ly6C-peridinin chlorophyll protein-Cy5.5, F4/80-PE-Cy7, and CD11b-eFluor 450, and viability was determined with a LIVE/DEAD Fixable Blue Dead Cell Stain (Life Technologies, Eugene, OR). All fluorochrome-conjugated Abs were purchased from BD Biosciences or eBioscience (San Diego, CA). An aliquot of cells was stained with isotype-matched control Abs to assess the degree of nonspecific staining. The number of events analyzed per sample ranged from 10,000 to 400,000. Analysis was performed with BD FACSDiva software with doublet exclusion performed, and cells were gated on the “live” CD45⁺ population.

Scherr T.D., Hanke M.L., Huang O., James D.B., Horswill A.R., Bayles K.W., Fey P.D., Torres V.J, & Kielian T. (2015). Staphylococcus aureus Biofilms Induce Macrophage Dysfunction Through Leukocidin AB and Alpha-Toxin. mBio, 6(4), e01021-15.

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Isolation and Sorting of Hes1+ Neural Stem Cells

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To isolated Hes1 positive neural stem cells (NSCs) at E14, dorsal cortices were dissected from Hes1-d2-EGFP Tg/+ mice. Cortices were dissociated with 0.05% trypsin with Hanks'Balanced Salt Solution (HBSS) (-) at 37 °C for 10 min. After centrifugation at 1000 g for 5 min, cells were resuspended with 0.375% BSA/HBSS(-) by gentle pipetting 15 to 20 times. Resuspended cells were filtered with 35 µm filter (Falcon) and sorted into sorting buffer (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco), in Dulbecco's Modified Eagle Medium (DMEM) F12+GlutaMax (Gibco)) by a cell sorter (SH800, SONY) equipped with 130 µm sorting chips (SONY, LE-C3113). After sorting, cell number was counted by Countess or Countess II (Invitrogen). For cells from wild type and Fbl mutant mice, cells were collected similarly except that sorting was not performed.
Collected cells were immediately load into the 10X-Genomics Chromium (10X Genomics, Pleasanton, CA). Libraries for single cell cDNA were prepared using Chromium 3′ v2 platform as the manufacturer′s protocol.

Wu Q., Shichino Y., Abe T., Suetsugu T., Omori A., Kiyonari H., Iwasaki S., & Matsuzaki F. (2020). Selective translation of epigenetic modifiers drives the developmental clock of neural stem cells.

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